Location:
Altensteinstr. 23a, room 114.3
Responsible Person:
- Stefanie Wedepohl, Arnimallee 22, E001, Tel. 58721, stefanie.wedepohl@fu-berlin.de
- Tatyana Povolotsky, Arnimallee 22, tatyana.povolotsky@fu-berlin.de
see OpenIRIS (protocls see here)
Type of measurement:
Liquid chromatography for protein purification/analysis in aqueous solutions at medium pressure (< 5 bar = 0.5 MPa) with automated fraction collector
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- All users must gain permission to work in the Biolabs and access to the “Zellbio-users” network drive by attending the Biosafety instructions (--> Katharina Achazi)
- All users must get an introduction to the device by the responsible person (see OpenIRIS)
- Booking via OpenIRIS (protocls see here)
- All buffers and solutions must be prepared with MilliQ, filtered (0,22µm) and degassed before use (simultaneous filtering and degassing can be done in E030)
- Read up on the properties of your column matrix (capacity, pore size, chemical resistance, max pressure, etc)
- Get familiar with the flow path
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