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Location:

Arnimallee 22, room E027Altensteinstr. 23a, 114


Responsible Person:

Stefanie Wedepohl, phone 58721, stefanie.wedepohl@fu-berlin.de

Katharina Achazi, phone: 59145,  katharina.achazi@fu-berlin.desee OpenIRIS (protocls see here)



Type of measurement:

The NanoDrop 2000 is a full-spectrum, UV-Vis spectrophotometers used to quantify and assess purity of DNA, RNA, Protein and more. It can measure 0.5- 2 ul samples with high accuracy and reproducibility.

The NanoDrop 2000c model offers the convenience of both the NanoDrop patented sample retention technology and a traditional cuvette for sample measurements. The sample retention system employs surface tension to hold the sample in place between two optical fibers. This enables the measurement of very highly concentrated samples without the need for dilutions. Using this technology, the full spectrum (190 – 840 nm) NanoDrop 2000/2000c Spectrophotometers have the capability to measure sample concentration up to 200 times more concentrated than samples measured using the standard cuvette.


Requirements:

  • all All users must gain permission to work in the Biolabs and access to the “Zellbio-users” network drive by attending the Biosafety instructions (--> Katharina Achazi)
  • all All users must get an introduction to the device by the responsible person mark the time slot at which you want to do a measurement in the calendar, to avoid conflict with other user’s plans(see OpenIRIS)
  • Booking via OpenIRIS (protocls see here)
  • clean the sample holder with water after use
  • don’t touch the computer keyboard and mouse with gloves.

...

Safety Operation Instruction (German):

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