Bacteria
E. coli BL21(DE) Ril
Strain/Type
K12
Characteristics e.g. sensitizing or toxic effects, resistance to antibiotics
Chloramphenicol resistance, E. coli B F– ompT hsdS(rB– mB–) dcm+Tetr galλ(DE3) endA Hte [argU ileY leuW Camr]
Approved as biological safety measure if taken as recipient organism for genetic engineering?
Genetically modified (GenTSV)
E. coli B F– ompT hsdS(rB– mB–) dcm+Tetr galλ(DE3) endA Hte [argU ileY leuW Camr] → S1
Risk group (BioStoffV)
Risk assessment
torage
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | amount of bacteria per vial | stock was produced on | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°C freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|
| Wahl Group | 03.11.21 | 100 µL | ZymoBroth | 15 | Robyn Diehn | B6 -80 °C | |
Background
Bacteria for heterologous protein production → has additional tRNAs for rare E. coli codons
