Cell Line (only for AG Weinhart !!!)
primary fibroblasts
Organism
human
Tissue/Cell type
foreskin
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Risk assessment
According to risk assessemnt for project Anzeige: Isolation von Zellen aus humanen Geweben und Körperflüssigkeiten
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| self-isolated from human foreskin samples | 8.2.2019 | p2 | 1000000 | DMEM High Glucose | 4 | Johanna Scholz | 13/10, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | donor 11 month, List no. A21, no detachment | ||||||
| self-isolated from human foreskin samples | 1.3.2019 | p2 | 1000000 | DMEM High Glucose | 3 | Johanna Scholz | 13/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | donor 11 years, List no. A23 cells growing slowly | ||||||
| self-isolated from human foreskin samples | 23.4.2019 | p2 | 1000000 | DMEM High Glucose | 1 | Johanna Scholz/Shabnam Hemmati | 06/02, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | donor 4 years, List no. A24 good detachment | ||||||
| self-isolated from human foreskin samples | 23.4.2019 | p2 | 1000000 | DMEM High Glucose | 7 | Johanna Scholz/Shabnam Hemmati | 06/02, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | donor 9 years, List no. A25 fast detachment | ||||||
| self-isolated from human foreskin samples | 04.10.2019 | p2 | 1000000 | DMEM High Glucose | 5 | Johanna Scholz | 06/02, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | donor 5 years, List no. A29 one hour detachment | ||||||
| self-isolated from human foreskin samples | 07.10.2019 | p3 | 1000000 | DMEM High Glucose | 6 | Johanna Scholz | 06/02, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | donor 1 year, List no. A30 fast detachment | ||||||
| self-isolated from human foreskin samples | 07.11.2019 | p2 | 1000000 | DMEM High Glucose | 2 | Johanna Scholz | 06/04, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | donor 9 years, List no. A31 | ||||||
| self-isolated from human foreskin samples Pool | 04.12.2019 | p3 | 800000-1000000 | DMEM High Glucose | 14 | Johanna Scholz | 06/04, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Pool of donor A21 +A25, List no.A32 good detachment | ||||||
| self-isolated from human foreskin samples | 06.12.2019 | p2 | 1000000 | DMEM High Glucose | 9 | Johanna Scholz | 06/04, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | donor 9 year, List no. A33 over one hour detachment | ||||||
| self-isolated from human foreskin samples | 28.02.2020 | p2 | 1000000 | DMEM High Glucose | 12 | Johanna Scholz | 06/02, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | donor 5 years, List no. A34 over one hour detachment | ||||||
| self-isolated from human foreskin samples | 23.03.2020 | p2 | 1000000 | DMEM High Glucose | 5 | Johanna Scholz | 06/04, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | donor 5 years, List no. A34 detachment 70-90 min | ||||||
| self-isolated from human foreskin samples | 05.09.2017 | p9 | 1000000 | DMEM 10% FBS 1% P/S | 17 | Stefanie Wedepohl | 05/04, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||||||
| self-isolated from human foreskin samples | 26.08.2015 | p3 | 1000000 | 1 | Anke Hoppensack | 13/06, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||||||||
| self-isolated from human foreskin samples | 19.10.2015 | p2 | 1000000 | 2 | Anke Hoppensack (Melanie Uckert) | 13/09, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||||||||
| self-isolated from human foreskin samples | 26.11.2015 | p2 | 1000000 | 1 | Anke Hoppensack (Melanie Uckert) | 13/09, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||||||||
| self-isolated from human foreskin samples | 26.11.2015 | p2 | 1000000 | 0 | Anke Hoppensack | 13/10, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | in FibroLife | |||||||
| self-isolated from human foreskin samples | 19.10.2016 | p1 | 1000000 | 4 | Anke Hoppensack (Laura Pleger) | 14/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||||||||
| self-isolated from human foreskin samples | 24/25.10.2016 | p2 | 1000000 | 13 | Anke Hoppensack (Laura Pleger) | 14/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||||||||
| self-isolated from human foreskin samples | 08.12.2016 | p2 | 1000000 | 5 | Anke Hoppensack | 14/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||||||||
| self-isolated from human foreskin samples | 26.09.2017 | p2 | 1000000 | 2 | Anke Hoppensack | 14/06, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||||||||
| self-isolated from human foreskin samples | 20.12.2016 | p2 | 1000000 | 3 | Anke Hoppensack | 14/06, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||||||||
| self-isolated from human foreskin samples | 06.12.2016 | p1 | 1000000 | 1 | Anke Hoppensack | 14/06, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||||||||
| self-isolated from human foreskin samples | 13.12.2016? | p2 | 1000000 | 12 | Anke Hoppensack (Laura Pleger) | 14/07, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||||||||
| self-isolated from human foreskin samples | 21.11.2017 | p1 | 1000000 | 1 | Johanna Scholz | 13/09, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||||||||
| self-isolated from human foreskin samples | 09.02.2018 | P3 | 1,40E+06 | DMEM 10% FBS 1% P/S | 10 | Stefanie Wedepohl | 05/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||||||
| self-isolated from human foreskin samples | 09.02.2018 | P5 | 1,40E+06 | DMEM 10% FBS 1% P/S | 10 | Stefanie Wedepohl | 05/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||||||
| self-isolated from human foreskin samples | 17.11.2020 | p2 | 1000000 | DMEM High Glucose | 3 | Johanna Scholz | 13/03, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||||||
| self-isolated from human foreskin samples | 08.02.2021 | p2 | 1000000 | DMEM High Glucose | 5 | Johanna Scholz | 06/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Spender 5 Jahre alt | ||||||
| self-isolated from human foreskin samples | 08.02.2021 | p3 | 1000000 | DMEM High Glucose | 9 | Johanna Scholz | 06/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Spender 6 Jahre alt | ||||||
| self-isolated from human foreskin samples | 25.2.21 | p2 | 1000000 | DMEM High Glucose | 6 | Johanna Scholz | 06/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Spender 4 Jahre alt | ||||||
| self-isolated from human foreskin samples | 24.2.21 | p2 | 1000000 | DMEM High Glucose | 4 | Johanna Scholz | 06/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Spender 1 Jahr alt für Angiogenesenur, für AG Weinhart, erst Johanna fragen | ||||||
| self-isolated from human foreskin samples | 27.4.21 | p4 | 1000000 | DMEM High Glucose | 2 | Johanna Scholz | 13/09, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Zellen vom 8.2.19 für Angiogenesenur für AG Weinhart | ||||||
| self-isolated from human foreskin samples | 19.7.21 | p4 | 1000000 | DMEM High Glucose | 4 | Johanna Scholz | 13/07, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Zellpool | ||||||
| self-isolated from human foreskin samples | 17.8.21 | p3 | 1000000 | DMEM High Glucose | 5 | Johanna Scholz | 06/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Zellpool 2 aus A31 und A25 hergestellt | ||||||
| self-isolated from human foreskin samples | 31.8.21 | p5 | 1000000 | DMEM High Glucose | 12 | Johanna Scholz | ? | Zellpool 2 | ||||||
| self-isolated from human foreskin samples | 20.12.21 | p2 | 1000000 | DMEM High Glucose | 6 | Johanna Scholz | 06/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Spender ist 6 Jahre alt, Zellen wachsen sehr langsam, Ablösen innerhalb von 30 Min. | ||||||
| self-isolated from human foreskin samples | 07.02.22 | p2 | 1000000 | DMEM High Glucose | 10 | Johanna Scholz | 06/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Spender ist 8 Jahre alt, Ablösen unter 1 Stunde, Mycoplasmenfrei | ||||||
| self-isolated from human foreskin samples | 14.2.22 | p4 | 1000000 | DMEM High Glucose | 4 | Johanna Scholz | 06/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Pool1 | ||||||
| self-isolated from human foreskin samples | 09.03.22 | p5 | 1000000 | DMEM High Glucose | 3 | Johanna Scholz | 13/09, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Pool1 | ||||||
| self-isolated from human foreskin samples | 31.5.22 | p3 | 1000000 | DMEM High Glucose | 3 | Johanna Scholz | 06/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | weitere Zellen vom 7.2.22, A48 | ||||||
| self-isolated from human foreskin samples | 10.10.22 | p3 | 1000000 | DMEM High Glucose | 3 | Johanna Scholz | 06/06, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Zellen vom 20.12.16 | ||||||
| self-isolated from human foreskin samples | DMEM High Glucose | Johanna Scholz | , N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) |
Background
Cell isolation carried out by AG Weinhart
Cultivation and freezing protocols
"USE ONLY IN AGREEMENT WITH AG WEINHART (Johanna Scholz or Anke Hoppensack)"
