Bacteria
Escherichia coli, E. coli
Strain/Type
derivative of E. coli K12 MG1655
Host tropism
Route of Transmission
Characteristics e.g. sensitizing or toxic effects, resistance to antibiotics
ΔtrpLEDC::FRT ΔtnaA::FRT (trp auxotrophic)
Approved as biological safety measure if taken as recipient organism for genetic engineering?
yes
Genetically modified (GenTSV)
deletions in trp operon (genes: trpL, trpD, trpE, trpC), deletion of tnaA gene (tryptophanase)
Risk group (BioStoffV)
1
Operation instructions (mandatory for RG2 and higher)
Occupational health care (according to ArbMedVV)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | bacterial strain | freezing date | amount of bacteria per vial | stock was produced on | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°C freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Technische Universität Berlin, group of Nediljko Budisa | 3 | Christin Treiber-Kleinke | -80°C "Omega", 115.2? | ancestral strain for Adaptive Laboratory Evolution; do not take/use it without my permission, please also available: TUB00 ECFP TUB00 EGFP | ||||
Background
trp auxotrophic strain; metabolic prototype able to synthetize Trp from supplemented indole; used as ancestral strain for several Adaptive laboratory evolution experiments
