Seitenhistorie

Bacteria

Pseudomonas aeruginosa

Strain/Type

Pseudomonas aeruginosaa: DSM-50071

Specification in other collections: ATCC 10145, ICPB 2523, NCIB 8295, NCTC 10332, NRRL B-771, WDCM 00024

Pseudomonas aeruginosa (Schroeter 1872) Migula 1900*

DSM No.: 939 

Strain designation: PRD-10

Other collection no. or WDCM no.: ATCC 15442, NCIB 10421

Pseudomonas aeruginosa (Schroeter 1872) Migula 1900*

DSM No.: 1128 

Other collection no. or WDCM no.: ATCC 9027, DSM 1385, CIP 82.118, WDCM 00026

Source/Company

*Prof. Dr. Elisabeth Grohmann, BHT, Fachbereich V Life Sciences und Technology, Labor für Mikrobiologie, Forum Seestraße, Seestraße 64, 2. OG, Raum 275, Tel.: +49 030 4504-3942

Usage Regulation and Limitations

*Acknowledge Prof. Dr. Elisabeth Grohmann (BHT, Fachbereich V Life Sciences und Technology, Labor für Mikrobiologie) in any publication.

How to cite

*Acknowledge Prof. Dr. Elisabeth Grohmann (BHT, Fachbereich V Life Sciences und Technology, Labor für Mikrobiologie) in any publication.

Host tropism

Very broad host spectrum.

There are no specific vectors.

Pseudomonas aeruginosa can be found in the intestines or on the mucous membranes of humans and animals as a commensal (colonising) agent. Hospital personnel may be involved in the spread of hospital strains without becoming ill themselves.

Route of Transmission

Transmission takes place percutaneously (through the skin).

Transmission takes place via inhalation (by breathe).

Zoonosis (transmission between animals and humans): Yes

Pseudomonas aeruginosa occurs ubiquitously in the environment, in soil, water bodies and on plants, especially in humid environments, in domestic and hospital settings also in toilets, showers, washbasins, flower vases, etc. Transmission occurs mainly through contact with contaminated media/objects. Transmission occurs mainly through contact with contaminated media/objects. Person-to-person transmission plays a role in hospitals and nursing homes. P. aeruginosa is one of the most frequent pathogens of nosocomial infections worldwide (especially pneumonia as well as wound and urinary tract infections).

Characteristics e.g. sensitizing or toxic effects, resistance to antibiotics

Pathogenicity:

Facultative human-pathogenic (it does not necessarily cause diseases in humans).

Facultative animal-pathogenic (it does not necessarily cause diseases in animals).

Toxigenicity/Toxin formation:

Pseudomonas aeruginosa produces exotoxin A, which inhibits protein synthesis in infected tissues.

Aside from this, toxic properties are ascribed to the pigment pyocyanin, which is secreted by Pseudomonas aeruginosa.

As a Gram-negative rod-shaped bacterium, Pseudomonas aeruginosa contains endotoxin (LPS).

Resistances:

Pseudomonas aeruginosa is naturally resistant to penicillin, macrolide antibiotics, folic acid antagonists and most aminoglycoside antibiotics. Pseudomonas aeruginosa is also resistant to various disinfectants in their diluted form.

Pseudomonas aeruginosa possesses a high degree of environmental resistance.

Note ht:

Pathogenic for humans and vertebrates, but normally no transmission between the two host groups.

Note T:

Toxin production: prokaryotes capable of forming exotoxins. The "T" tag lays no claim to completeness, however, i.e. exotoxin-forming strains may also arise in types of prokaryote without this tag. The "T" tag adopted over from Annex III of Directive 2000/54/EC.

Approved as biological safety measure if taken as recipient organism for genetic engineering?

Genetically modified (GenTSV)

Risk group (BioStoffV)

2

Risk assessment

Risk accessment based on TRBA (Technical Rule for Biological Agents) 466 "Classification of prokaryotes (bacteria and archaea) into risk groups": https://www.baua.de/DE/Angebote/Rechtstexte-und-Technische-Regeln/Regelwerk/TRBA/TRBA-466.html

Operation instructions (mandatory for RG2 and higher)

https://bioagent.dguv.de/data?name=825301

Occupational health care (according to ArbMedVV)

Optional health care:
In the case of tasks specifically involving contact and tasks involving incidental contact with biological agents classed as Risk Group 2 under the Biological Agents Ordinance (Biostoffverordnung, BioStoffV) or which involve a comparable risk, the employer must offer an optional health care. This does not apply when on account of the risk assessment and on account of the protective measures taken it can be assumed that there is no risk of infection.
An optional health care must also be offered if as a result of the exposure to biological agents
- a serious infectious illness is to be expected and post-exposure prophylatic measures are possible, or
- an infection has resulted.

Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)

Background

https://www.dsmz.de/collection/catalogue/details/culture/dsm-939

https://www.dsmz.de/collection/catalogue/details/culture/DSM-1128

Cultivation and freezing protocols

DSM 939

Medium 92 , 30°C
or
Medium 693 , 37°C
Incubation time: 1-2 days
Complete DSMZ Media List

DSM 1128

Medium 1 , 37°C
or
Medium 693 , 37°C
or
Medium 92 , 37°C
Incubation time: 1-2 days
Complete DSMZ Media List

Data sheets, further information