Bacteria incl. Archaea
E. coli BL21(DE3)
Strain/Type
E.coli B
Source/Company
AG Hackenberger/New England Biolabs
Usage Regulation and Limitations
Ask Robyn (AG Lauster)
How to cite
Host tropism
Route of Transmission
Characteristics e.g. sensitizing or toxic effects, resistance to antibiotics
No antibiotic resistance
Approved as biological safety measure if taken as recipient organism for genetic engineering?
As risk group 1
Genetically modified (GenTSV)
BL and DE3 modification → S1
Risk group (BioStoffV)
S1
Risk assessment
Operation instructions (mandatory for RG2 and higher)
Occupational health care (according to ArbMedVV)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | bacterial strain | freezing date | amount of bacteria per vial | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°C freezer and location (room, address) | comments | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AG Hackenberger/New England Biolabs | E. coli BL21(DE3) | 26.04.24 | 100 µL | 15 | Robyn Diehn | -80°C "Omega", 115.2 B-2-2 | chemical competent | ||||||||||||
| E. coli BL21(DE3) RIL from AG Wahl | E. coli BL21(DE3) | 16.04.24 | 100 µL | 15 | Robyn Diehn | -80°C "Omega", 115.2 B-2-3 | chemical competent, made by growing E. coli BL21(DE3) Ril until chloramphenicol resistance got lost | ||||||||||||
| AG Hackenberger/New England Biolabs | E. coli BL21(DE3) | 13.05.24 | 2 mL | 3 | Robyn Diehn | -80°C "Omega", 115.2 B-2-20 | Plasmid inside: P6TV13_OmpA_C04_Di_GG | ||||||||||||
| E. coli BL21(DE3) RIL from AG Wahl | E. coli BL21(DE3) | 500 µL | 5 | Robyn Diehn | -80°C "Omega", 115.2 B-2-8 | stock, made by growing E. coli BL21(DE3) Ril until chloramphenicol resistance got lost | |||||||||||||
| E. coli BL21(DE3) RIL from AG Wahl | E. coli BL21(DE3) | 26.04.24 | 1 mL | 4 | Robyn Diehn | -80°C "Omega", 115.2 B-2-8 | stock, made by growing E. coli BL21(DE3) Ril until chloramphenicol resistance got lost | ||||||||||||
| AG Hackenberger/New England Biolabs | E. coli BL21(DE3) | 1 mL | 4 | Robyn Diehn | -80°C "Omega", 115.2 B-2-8 | stock | |||||||||||||
Background
For expression of T7 promoter plasmids
