Bacteria
Escherichia coli
Strain/Type
E. coli DH10B, K12 derivative
Genotype: str. K-12 F– Δ(ara-leu)7697[Δ(rapA'-cra' )] Δ(lac)X74[Δ('yahH-mhpE)] duplication(514341-627601)[nmpC-gltI] galK16 galE15 e14–(icdWT mcrA) φ80dlacZΔM15 recA1 relA1 endA1 Tn10.10 nupG rpsL150(StrR) rph+ spoT1 Δ(mrr-hsdRMS-mcrBC) λ– Missense(dnaA glmS glyQ lpxK mreC murA)
Nonsense(chiA gatZ fhuA? yigA ygcG) Frameshift(flhC mglA fruB)
Characteristics e.g. sensitizing or toxic effect, resistance to antibiotics
F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG λ-
Approved as biological safety measure if taken as recipient organism for genetic engineering?
yes
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/ecoli/detail.jsf?id=16&dswid=9548&dsrid=69)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | bacterial strain | freezing date | amount of bacteria per vial | host cells, stock was produced on | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments | |
|---|---|---|---|---|---|---|---|---|---|
| Welcome Sanger Institute | Suman Chowdhury | minus 80°C freezer (Arnimallee 22, E004) | -80°C "Omega", 115.2? | ||||||
Background
An MC1061 derivative (Casadaban80). Prepare cells for chemical transformation with CCMB80 buffer
Thermo TOP10 and NEB 10-beta commercial strains are close derivatives
Recombination-deficient (recA1)
Endonuclease I-deficient (endA1)
Relaxed phenotype (relA1)
Constitutive deoxyribose synthesis for improving cloning of large plasmids
Suitable for cloning methylated cytosine or adenine containing DNA
High competence (galE–)
Blue/white screening with lacZα.
(galE galK galU)+
deoR+
Streptomycin-resistant and 2-deoxygalactose-resistant
Leucine auxotroph
Arabinose, lactose, and galactose non-utilizing
Background