Bacteria
Escherichia coli
Strain/Type
E. coli DH5a, K12 derivative
Genotype: F– endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK–mK+), λ–
Characteristics e.g. sensitizing or toxic effect, resistance to antibiotics
F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ–
Approved as biological safety measure if taken as recipient organism for genetic engineering?
yes
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/ecoli/detail.jsf?id=17&dswid=2647&dsrid=990)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | bacterial strain | freezing date | amount of bacteria per vial |
|---|
| no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments | ||
|---|---|---|---|---|---|
| Invitrogen | Mingjun Li/ Stefanie Wedepohl |
| -80°C "Omega", 115.2? | |||||||
| 1 ml | 24 | Elisa Quaas | -80°C "Omega", Shelf B, Rack 1, Box 5, Room 115.2, SupraFAB, Altensteinstraße 23a | ||||
| Mingjun Li | 1ml | 5 | Chuanxiong Nie | -80°C "Π", A-5-31 | |||
| Elisa Quaas | 1 mL | 1 | Natalie Hanheiser | -80°C "Omega", Shelf B, Rack 1, Room 115.2, SupraFAB, Altensteinstraße 23a | |||
| Elisa Quaas | E. coli DH5α | 1 mL | 1 | Tatyana Povolotsky | -80°C "Omega", 115.2 B-2-11 | Frozen stock strain | |
| Addgene | E. coli DH5α | 1 mL | 1 | Tatyana Povolotsky | -80°C "Omega", 115.2 B-2-11 | EGFP pBAD in E. coli DH5α |
Background
A Hoffman-Berling 1100 strain derivative (Meselson68)
Thiamine, arginine auxotroph
Lactose nonutilizing
Nalidixic acid resistant
Recombination-deficient
Endonuclease A deficient
Relaxed phenotype
Constitutive deoxyribose synthesis, good for cloning large plasmids
Amber suppressor UAG>CAG(Gln)
Slow growth in M9 medium due to purB-20
Background