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Bacteria

Escherichia coli

Strain/Type

E. coli DH5a, K12 derivative

Characteristics e.g. sensitizing or toxic effect, resistance to antibiotics

F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Genotype: F φ80lacZΔM15 Δ(lacZYA-argF)U169 , hsdR17recA1 endA1 hsdR17(rK- , mK+) , λ–phoA supE44 λthi-1 gyrA96 relA1

Characteristics e.g. sensitizing or toxic effect, resistance to antibiotics

 

Approved as biological safety measure if taken as recipient organism for genetic engineering?

yes

Genetically modified (GenTSV)

Risk group (BioStoffV)

1

Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)

sourcebacterial strainfreezing dateamount of bacteria  per vial
host cells, stock was produced on
no. of aliquotsbelongs to
(full name)

rack/box in N2 tank or -80°° freezer and  location (room, address)


comments

Invitrogen/

(Charité)

E. coli DH5α21.1.2016nd1Mingjun Li/ Stefanie Wedepohl
minus 80°C freezer (Arnimallee 22, E004)

-80°C "Omega", 115.2

Shelf B Rack 6 Box 1, I1

glycerol stock

Invitrogen/

(Charité)

E. coli DH5α21.1.2016100 µL
var.Stefanie Wedepohl

-80°C "Omega", 115.2

Shelf B Rack 6 Box 1, row 6 and 7

aliquots of chemically competent bacteria prepared from glycerol stock



1 ml24Elisa Quaas-80°C "Omega", Shelf B, Rack 1, Box 5, Room 115.2, SupraFAB, Altensteinstraße 23a
Mingjun Li

1ml5Chuanxiong Nie-80°C "Π", A-5-31
Elisa Quaas 

1 mL1Natalie Hanheiser -80°C "Omega", Shelf B, Rack 1,  Room 115.2, SupraFAB, Altensteinstraße 23a
Elisa QuaasE. coli DH5α
1 mL1Tatyana Povolotsky-80°C "Omega", 115.2 B-2-11

Frozen stock strain

AddgeneE. coli DH5α
1 mL1Tatyana Povolotsky-80°C "Omega", 115.2 B-2-11

EGFP pBAD in E. coli DH5α


Background

A Hoffman-Berling 1100 strain derivative (Meselson68)

Thiamine, arginine auxotroph

Lactose nonutilizing

Nalidixic acid resistant

Recombination-deficient

Endonuclease A deficient

Relaxed phenotype

Constitutive deoxyribose synthesis, good for cloning large plasmids

Amber suppressor UAG>CAG(Gln)

Slow growth in M9 medium due to purB-20

Cultivation and freezing protocols

View file
namePreparing chemically competent E.docx
height250

Data sheets, further information