Seitenhistorie

Cell Line

HEK293T; 293T; HEK 293T; HEK-293T; HEK-293-T; HEK 293 T; 293-T; 293 T; Human Embryonic Kidney 293T; 293tsA1609neo

Organism

human

Tissue/Cell type

human embryonic kidney

Genetically modified (GenTSV)

S1

Risk group (BioStoffV)

1

Risk assessment

According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=2733&dswid=9596&dsrid=863)

Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)

source	freezing date	passage no.	cell count per vial	cell culture medium incl. supplements	no. of aliquots	belongs to (full name)	rack/box in N2 tank or -80°° freezer and location (room, address)	comments
DSMZ	25.02.2018	p4		DMEM, 10%FCS	20	Karen Vester, AG Wahl	N2 tank (Takustr. 6, K004)
	21.06.2019		1,00E+06	DMEM 10% FBS 1% P/S	8	Katharina Achazi
	20.04.2016				9	Yan Li, AG Ma (Katharina Achazi)	03/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)
Jens Dernedde, Charite (DSMZ)	30.07.2013	px+1			4	Katharina Achazi (CK)	05/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)
Uni Leipzig, Michael Schäfer (DSMZ)	29.10.2014	p3/p4			6	Katharina Achazi/Andrea Senger, FU Biochemie	05/03, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)	WT
AG Knaus (DSMZ)	30.10.2014				8	Katharina Achazi/Andrea Senger, FU Biochemie	05/03, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)	WT
Jens Dernedde, Charite (DSMZ)	08.04.2014	px+3			5	Katharina Achazi (CK)	12/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)
Jens Dernedde, Charite (DSMZ)	12.11.2014	px+2			14	Katharina Achazi (CK)	12/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)
Jens Dernedde, Charite (DSMZ)	26.10.2016	px+9	for 25cm2 flask	DMEM (4.5g/l glucose), 10% FBCS, 1% P/S	7	Katharina Achazi	04/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)	Does not exist anymore?
DSMZ	15.04.2019			DMEM 10% FBS 1% P/S	5	Stefanie Wedepohl	05/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)
DSMZ	17.11.2021			DMEM 10% FBS 1% P/S	4	Chuanxiong Nie	04/07, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)
DSMZ				DMEM 10% FBS 1% P/S	2	Chuanxiong Nie	6/9, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)
DSMZ	11.14.2023		1.8x10^6 cells	DMEM 10% FBS 1% P/S	8	Chuanxiong Nie	6/9, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)
FU Insitut Fur Virologie	03.04.2024	px+1	1x10^6 cells	DMEM 10% FBS 1% P/S	6	Chuanxiong Nie	6/8, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)
Daniel Lauster	22.05.22	P7	0.5x10^5 cells	DMEM 5% FBS 1% P/S	3	Robyn Diehn	10/07, N2 Tank ((SupraFab, Altensteinstraße 23a, 114.1)	Suspension
Robyn Diehn	12.07.22	P8	2x10^5 cells	DMEM 1.25% FBS 1% P/S	4	Robyn Diehn	10/07, N2 Tank ((SupraFab, Altensteinstraße 23a, 114.1)	Suspension
Robyn Diehn	12.10.22	P4	2.5x10^6 cells	DMEM 1.25% FBS 10% P/S	3	Robyn Diehn	10/07, N2 Tank ((SupraFab, Altensteinstraße 23a, 114.1)	Adherent
Robyn Diehn	14.10.22	P4	6x10^6 cells	9:1 Freestyle/ExCell medium 140 rpm, 1% P/S, 8% CO2	3	Robyn Diehn	10/08, N2 Tank ((SupraFab, Altensteinstraße 23a, 114.1)	Suspension
Robyn Diehn	18.10.22	P6	2.5x10^6 cells	DMEM, 10% FBS, 1% P/S	3	Robyn Diehn	10/08, N2 Tank ((SupraFab, Altensteinstraße 23a, 114.1)	Adherent
Robyn Diehn	24.01.23	P10	6x10^6 cells	9:1 Freestyle/ExCell medium 140 rpm, 1% P/S, 8% CO2	3	Robyn Diehn	10/08, N2 Tank ((SupraFab, Altensteinstraße 23a, 114.1)	Suspension
Robyn Diehn	01.02.23	P12	6x10^6 cells	9:1 Freestyle/ExCell medium 140 rpm, 1% P/S, 8% CO2	3	Robyn Diehn	10/08, N2 Tank ((SupraFab, Altensteinstraße 23a, 114.1)	Suspension
Robyn Diehn	15.02.23	P18	6x10^6 cells	9:1 Freestyle/ExCell medium 140 rpm, 1% P/S, 8% CO2	3	Robyn Diehn	10/08, N2 Tank ((SupraFab, Altensteinstraße 23a, 114.1)	Suspension
Robyn Diehn	22.02.23	P21	6x10^6 cells	9:1 Freestyle/ExCell medium 140 rpm, 1% P/S, 8% CO2	4	Robyn Diehn	10/08, N2 Tank ((SupraFab, Altensteinstraße 23a, 114.1)	Suspension