Cell Line
A549; A-549; A 549; NCI-A549; A549/ATCC; A549 ATCC; A549ATCC; hA549
Organism
human
Tissue/Cell type
lung adenocarcinoma, lung carcinoma
Genetically modified (GenTSV)
Risk group (BioStoffV), sensitizing or toxic effects
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=416&dswid=1649&dsrid=736)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
Cultivation and freezing protocols
check data sheet
Data sheets, further information
https://www.dsmz.de/collection/catalogue/details/culture/ACC-107
https://www.lgcstandards-atcc.org/products/all/CCL-185.aspx?geo_country=dec
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| DSMZ | 06.12.2017 | px+2 | DMEM, 10% FCS | 1 | Katharina Achazi (Jing Li) | 11/05, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | ||
| DSMZ | 26.03.2018 | px+4 | DMEM, 10% FCS | 5 | Chuanxiong Nie | 6/9, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | ||
| DSMZ | 28.08.2012 | p8 | DMEM, 10% FCS | 5 | Kathariina Achazi | 05/01, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | ||
| DSMZ | 15.10.2012 | p9 | DMEM, 10% FCS | 20 | Kathariina Achazi | 05/01, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | ||
| DSMZ | 28.02.2019 | p16 | 100000 | DMEM, 10% FCS | 3 | Elisa Quaas/Katharina Achazi | 05/01, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | 21.10.2019 | p27 | 100000 | DMEM, 10% FCS | 63 | Elisa Quaas | 05/02, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | 16.12.2013 | p37 | DMEM 10% FBS 1% P/S | 2 | Stefanie Wedepohl | 05/04, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | ||
| DSMZ | 15.04.2013 | p9 | DMEM 10% FBS 1% P/S | 2 | Stefanie Wedepohl | 05/04, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | ||
| DSMZ | 22.04.2014 | p14 | DMEM 10% FBS 1% P/S | 8 | Stefanie Wedepohl | 05/04, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | ||
| DSMZ | 15.09.2014 | p3 | DMEM, 10% FCS | 5 | Kathariina Achazi (CK) | 12/02, N2 tank (Arnimallee 22, E004) | ||
| DSMZ | 10.10.2014 | p9 | DMEM, 10% FCS | 16 | Katharina Achazi (CK) | 12/03, N2 tank (Arnimallee 22, E004) | ||
| DSMZ | 20.01.2014 | p12 | DMEM, 10% FCS | 16 | Katharina Achazi (CK) | 12/09, N2 tank (Arnimallee 22, E004) | ||
| DSMZ | 25.06.2015 | DMEM, 10% FCS | 10 | Katharina Achazi (Wei) | 13/02, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |||
| DSMZ | 26.10.2016 | px+5 | for 25cm2 flask | DMEM (4.5g/l glucose), 10% FBCS, 1% P/S | 3 | Katharina Achazi | 04/01, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | tested mycoplasm free before freezing |
| DSMZ | 07.06.2016 | p8 | 1,08E+06 | DMEM | 8 | Katharina Achazi (PH) | 03/01, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | 06.07.2017 | px+13 | 100000 | DMEM | 24 | Katharina Achazi (NR) | 14/09, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | 06.07.2017 | px+13 | 100000 | DMEM | 8 | Katharina Achazi (NR) | 14/10, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | 30.04.2017 | 9 | Yan Li, AG Ma (Katharina Achazi) | 03/10, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | ||||
| DSMZ | 09.02.2018 | Px+7 | 1,00E+06 | DMEM (4.5g/l glucose), 10% FBS, 1% P/S | 6 | Stefanie Wedepohl | 05/05, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | 30.04.2017 | 1000000 | DMEM | 8 | Jie Chen (Peng Tang) | 01/07, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | ||
| KSMZ | 17.06.2023 | Px+2 | 1,00E+06 | DMEM | 2 | Yuhang Jiang | 04/09, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | 08.2018 | px | DMEM (4.5g/l glucose), 10% FBS, 1% P/S | 43 | Chuanxiong Nie | 06/09, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) |
| DSMZ |
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Cell Line
A549; A-549; A 549; NCI-A549; A549/ATCC; A549 ATCC; A549ATCC; hA549
Organism
human
Tissue/Cell type
lung adenocarcinoma, lung carcinoma
Genetically modified (GenTSV)
Risk level (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=416&dswid=1649&dsrid=736)
| 30.01.2024 | PX1 | 1,00E+06 | DMEM ( |
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| DSMZ | 06.12.2017 | px+2 | DMEM, 10% FCS | 1 | Katharina Achazi (Jing Li) | 11/05, N2 tank (Arnimallee 22, E004) | DSMZ | 26.03.2018 | px+4 | DMEM, 10% FCS | 5 | Chuanxiong Nie | 11/01, N2 tank (Arnimallee 22, E004) | DSMZ | 28.08.2012 | p8 | DMEM, 10% FCS | 5 | Kathariina Achazi | 05/01, N2 tank (Arnimallee 22, E004) | DSMZ | 15.10.2012 | p9 | DMEM, 10% FCS | 20 | Kathariina Achazi | 05/01, N2 tank (Arnimallee 22, E004) | DSMZ | 28.02.2019 | p16 | 100000 | DMEM, 10% FCS | 4 | Elisa Quaas/Katharina Achazi | 05/01, N2 tank (Arnimallee 22, E004) | DSMZ | 21.10.2019 | p27 | 100000 | DMEM, 10% FCS | 11 | Elisa Quaas | 05/02, N2 tank (Arnimallee 22, E004) | DSMZ | 16.12.2013 | p37 | DMEM 10% FBS 1% P/S | 2 | Stefanie Wedepohl | 05/04, N2 tank (Arnimallee 22, E004) | DSMZ | 15.04.2013 | p9 | DMEM 10% FBS 1% P/S | 2 | Stefanie Wedepohl | 05/04, N2 tank (Arnimallee 22, E004) | DSMZ | 22.04.2014 | p14 | DMEM 10% FBS 1% P/S | 8 | Stefanie Wedepohl | 05/04, N2 tank (Arnimallee 22, E004) | DSMZ | 15.09.2014 | p3 | DMEM, 10% FCS | 5 | Kathariina Achazi (CK) | 12/02, N2 tank (Arnimallee 22, E004) | DSMZ | 10.10.2014 | p9 | DMEM, 10% FCS | 16 | Katharina Achazi (CK) | 12/03, N2 tank (Arnimallee 22, E004) | DSMZ | 20.01.2014 | p12 | DMEM, 10% FCS | 16 | Katharina Achazi (CK) | 12/09, N2 tank (Arnimallee 22, E004) | DSMZ | 25.06.2015 | DMEM, 10% FCS | 10 | Katharina Achazi (Wei) | 13/02, N2 tank (Arnimallee 22, E004) | DSMZ | 26.10.2016 | px+5 | for 25cm2 flask | DMEM (4.5g/l glucose), 10% | FBCSFBS, 1% P/S | 3 | Katharina Achazi | 04/01, N2 tank (Arnimallee 22, E004) | tested mycoplasm free before freezing | 9 | Jingyi Tang | 06 | DSMZ | 07.06.2016 | p8 | 1,08E+06 | DMEM | 8 | Katharina Achazi (PH) | 03/01, N2 tank (Arnimallee 22, E004) | DSMZ | 06.07.2017 | px+13 | 100000 | DMEM | 24 | Katharina Achazi (NR) | 14/09, N2 tank (Arnimallee 22, E004) | DSMZ | 06.07.2017 | px+13 | 100000 | DMEM | 8 | Katharina Achazi (NR) | 14/10, N2 tank | (Arnimallee 22, E004)DSMZ | 30.04.2017 | 9Yan Li, AG Ma (Katharina Achazi) | 03/10, N2 tank (Arnimallee 22, E004) | DSMZ | 09.02.2018 | Px+7 | 1,00E+06 | DMEM (4.5g/l glucose), 10% FBS, 1% P/S | 6 | Stefanie Wedepohl | 05/05, N2 tank (Arnimallee 22, E004) | (SupraFab, Altensteinstraße 23a, 114.1) |
Cultivation and freezing protocols
check data sheet
Data sheets, further information
https://www.dsmz.de/collection/catalogue/details/culture/ACC-107
https://www.lgcstandards-atcc.org/products/all/CCL-185.aspx?geo_country=dec
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