Cell Line
HuH-7; HUH-7; HuH7; Huh7; HUH7; HUH7.0; JTC-39; Japanese Tissue Culture-39
Organism
Human
Source/Company and accesion number (article number)
Charité Universitaetsmedizin Berlin, Christian Drosten, CVCL_0336
Usage Regulation and Limitations
Obey the usage regulations listed in the MTA including that the virus can only be used for the reseach project listed in the MTA and it is not for use in anaimal experiments:
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How to cite
Refer for publications to the rules listed in the MTA including that the Charité need to be informed 4 weeks prior to a publication and the Chharité and Christian Drosten need to be either co-author or acknowledged:
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Tissue/Cell type
Hepatocytes
Genetically modified (GenTSV)
Risk group (BioStoffV), sensitizing or toxic effects
1
Risk assessment
according to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?dswid=9500&dsrid=940&id=25)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°C freezer and location (room, address) | comments | |
|---|---|---|---|---|---|---|---|---|---|
| Charité Universitaetsmedizin Berlin, Christian Drosten | 26.3.2024 | P11 | 1 mio | DMSO 20% FBS 80% | 10 * 500µl | Katharina Achazi/Chuanxiong Nie | N2 tank | in preaprationRack 7 Box 5 | |
| Charité Universitaetsmedizin Berlin, Christian Drosten | 02.04.2024 | P15 | 1 mio | DMEM medium + 10% DMSO | 5*1ml | Chuanxiong Nie | N2 tank Π (6/8, room 114, Altensteinstr. 23a) |
Background
Cultivation and freezing protocols
Data sheets, further information
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