Bacteria
Escherichia coli, E.coli
Strain/Type
E. coli B derivative (BL21(DE3))
Host tropism
Route of Transmission
Characteristics e.g. sensitizing or toxic effects, resistance to antibiotics
F– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS)
Approved as biological safety measure if taken as recipient organism for genetic engineering?
no
Genetically modified (GenTSV)
yes (S1): pQE80L H6-TrpRS (3 different variants)
Risk group (BioStoffV)
1
Operation instructions (mandatory for RG2 and higher)
Occupational health care (according to ArbMedVV)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | bacterial strain | freezing date | amount of bacteria per vial | stock was produced on | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°C freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Transformation (conducted by Christin Treiber-Kleinke) | 3 | Christin Treiber-Kleinke | -80°C "Omega", 115.2? | 3 different variants: TrpRS wt TrpRS Q27P TrpRS L25L Q27P | ||||
Background
expression of TrpRS variants (wt and mutated in ALEs)
