Influenza A Virus LPAIV (RG2)

wetVirus

Strain/Type

Genus: Influenzavirus A

Strains in SupraFAB room 114:

Sialic acid specificity of HA and NA may change upon propagating host

H3N2:

X31: A/Aichi/2/68 (H3N2) reassorted with internal segments of A/Puerto Rico/8/1934, chicken egg propagated

A/Panama/2007/1999, MDCK-II propageted

A/mallard/Germany/439/2004, chicken egg propageted

A/udorn/1972, MDCK-II propageted3

H1N1:

A/Puerto Rico/8/1934, chicken egg propageted

A/WSN/1933, chicken egg propagated

A/New Caledonia/02/99, chicken egg propageted

A/Hamburg/02/2009; chicken egg propageted

A/Bayern/63/2009, chicken egg propageted

A/swine/Wisconsin/1/76,  chicken egg propageted

H7N1-LP:

A/mute swan/Rostock/R901/2006 (LP, low pathogenic)

H7N7-LP:

A/turkey/Germany/R11/2001 (low pathogenic)

H5N1:

A/Teal/Germany/2005 (H5N1; HA und NA Genen von A/Teal/Germany/2005, reassorted with A/WSN/1933 (H1N1), MDCK-II propagated

H12N5:

A/Duck/Alberta/76,  chicken egg propageted

Host tropism

Humans, pigs, horses and other animal species.

Human (infecting humans) and avian (infecting birds) influenza viruses have different receptors. Pigs have receptors for both types of influenza.

This biological agent is host-dependent parasitical.

Route of Transmission

Transmission takes place via inhalation (by breathe).

Transmission takes place orally (by ingestion).

Zoonosis (transmission between animals and humans): Yes

Characteristics e.g. sensitizing or toxic effects, replication deficiant, not infectious, attenuated, inactivated (upload relevant documents)

Human-pathogenic (causes diseases in humans).

Footnote j:

Influenza A viruses are characterised by high diversity, which is defined by 16 haemagglutinin and 9 neuraminidase subtypes. The natural host species for these viruses are the waterfowl. Influenza A viruses can be transferred to other animal species and have formed new lines for humans, pigs, horses, dogs and seals. The sporadic transmission of avian influenza A virus without the formation of new lines has been observed in anteater, muskrat, ferret, camel, cat, leopard, humans, mink, pila, stone marten, tiger, whale, racoon, racoon dog and civet cat.

Approved as biological safety measure if taken as recipient organism for genetic engineering?

Genetically modified (GenTSV)

Risk group (BioStoffV)

2

Risk assessment

Risk accessment based on TRBA (Technical Rule for Biological Agents) 462 "Classification of viruses into risk groups": https://www.baua.de/DE/Angebote/Rechtstexte-und-Technische-Regeln/Regelwerk/TRBA/TRBA-462.html

Operation instructions (mandatory for RG2 and higher)

https://biostoffe.dguv.de/data?name=830658&lang=en

Occupational health care (according to ArbMedVV)

Optional health care:
In the case of tasks specifically involving contact and tasks involving incidental contact with biological agents classed as Risk Group 2 under the Biological Agents Ordinance (Biostoffverordnung, BioStoffV) or which involve a comparable risk, the employer must offer an optional health care. This does not apply when on account of the risk assessment and on account of the protective measures taken it can be assumed that there is no risk of infection.
An optional health care must also be offered if as a result of the exposure to biological agents
- a serious infectious illness is to be expected and post-exposure prophylatic measures are possible, or
- an infection has resulted.

Vaccination:

Available against seasonal flu. 

Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)

Background

Cultivation and freezing protocols

Data sheets, inactivation cetificate, further information