Cell Line
VeroE6/TMPRSS2
Organism
Chlorocebus aethiops - Grivet monkey
Source/Company and accession number (article number)
NIBSC via Prof. Dr. Benedikt Kaufer and Dr. Jakob (Institute of Virology, Department of Veterinary Medicine, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13)
Catalogue Number 100978
Cellosaurus Accession Number: CVCL_YQ49
Usage Regulation and Limitations
For publication, always include Prof. Dr. Benedikt Kaufer and Dr. Jakob (Institute of Virology, Department of Veterinary Medicine, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13) as co-authors!
The acknowledgment should read: “The cell line VeroE6/TMPRSS2 was provided by the NIBSC Research Reagent Repository, UK. With thanks to Dr. Makoto Takeda and donated to CFAR from the Japanese Collection of Research Bioresources Cell Bank (JCRB), National Institutes of Biomedical
Innovation, Health and Nutrition.”
Please also ensure that you send us a copy of any papers resulting from work using VeroE6/TMPRSS2 acquired through CFAR to the Resource Manager at the address given below:
Resource Manager, Reagent Repository
Division of lnfectious Disease Diagnostics
National Institute for Biological Standards and Control
Blanche Lane, South Mimms
Potters Bar, Herts, EN6 3QG
United Kingdom
Or emailed to: CFAR@nibsc.org
For details, read the MTA and data sheet carefully:
How to cite
To cite this cell line use the name VeroE6/TMPRSS2 (RRID:CVCL_YQ49) and refer to the following publications:
Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.
Matsuyama S et.al. Proc Natl Acad Sci U S A. 2020 Mar 31;117(13):7001-
7003
Consensus and variations in cell line specificity among human
metapneumovirus strains. Nao N e. al. PLoS One. 2019;14(4):e0215822
Tissue/Cell type
Kidney cells of Chlorocebus aethiops - Grivet monkey
Transfer is subject to CITES regulations.
Genetically modified (GenTSV)
yes
The VeroE6 cell line has been modified to constitutively express the serine protease TMPRSS2, under selection of geneticin. The resulting VeroE6/TMPRSS2 cells were shown to be highly susceptible to SARS-CoV-2 infection and can be used to produce large viral stock.
Risk group (BioStoffV), sensitizing or toxic effects
1
Risk assessment
The VeroE6 cell line has been modified to constitutively express the serine protease TMPRSS2, under selection of geneticin.
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°C freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Vet Department FU from NIBSC | 2024.02.15 | P2 | 10^6 cells | MEM, 10% FBS, 1% P/S, 1% NEAA, 1% Sodium pyruvate | 6 | Chuanxiong Nie | 6/8, N2 tank, room 114, Altensteinstr. 23a | |
| Vet Department FU from NIBSC | 2024.02.18 | P3 | 5x10^5 cells | MEM, 10% FBS, 1% P/S, 1% NEAA, 1% Sodium pyruvate | 4 | Chuanxiong Nie | 6/8, N2 tank, room 114, Altensteinstr. 23a |
Background
Cultivation and freezing protocols
Cultivation: DMEM, 10% (Heat Inactivated) FBS, 2mM Glutamine, 1mg/ml Geneticin (G418))
Freezing: DMEM, 20% FBS, 1% P/S, 1% NEAA, 1% Sodium pyruvate + 10% DMSO
