Bacteria
Escherichia coli, E.coli
Strain/Type
derivative of E.coli K12 TUB00
Host tropism
Route of Transmission
Characteristics e.g. sensitizing or toxic effects, resistance to antibiotics
ΔtrpLEDC::FRT ΔtnaA::FRT (trp auxotrophic), hypermutator strain (WGS data available on demand)
Approved as biological safety measure if taken as recipient organism for genetic engineering?
yes
Genetically modified (GenTSV)
deletions in trp operon (genes: trpL, trpD, trpE, trpC), deletion of tnaA gene (tryptophanase)
Risk group (BioStoffV)
1
Operation instructions (mandatory for RG2 and higher)
Occupational health care (according to ArbMedVV)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | bacterial strain | freezing date | amount of bacteria per vial | stock was produced on | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°C freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| evolved through adaptive laboratory evolution (conducted by Christin Treiber-Kleinke) | 3 | Christin Treiber-Kleinke | -80°C "Omega", 115.2
| 6Fi ALE experiment; do not take/use it without my permission, please 165 passages available of the 6TUB165-MB4 lineage (in biological triplicates) Subpopulation ALE: lineages 6TUB126-SP - 6TUB154-SP (clones 35, 49, 60) also available: 6TUB165-MB4 pQE80L H6 ECFP 6TUB165-MB4 pQE80L EGFP H6 | ||||
Background
6Fi ALE experiment, adapted to grow on 6-fluoroindole, 165 passages, cAA removal by MB approach (metabolic blocks)
