Cell Line
HeLa/GFP
Organism
human
Tissue/Cell type
cervix carcinoma, adeno carcinoma, epithelial
Genetically modified (GenTSV)
Yes, S1: GFP and blasticidin-resistant genes are introduced into parental HeLa cells using lentivirus
Risk group (BioStoffV), sensitizing or toxic effects
1
Risk assessment
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°C freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| BioCat Company | 13.10.2023 | px+5 | 1.4 x 106 | DMEM (10% FBS, 1% P/S, 0,1 mM NEAA, 10µg/mL Blasticidin) | 8 | Boyu Zheng | Rack 10/04, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| BioCat Company | 26.10.2023 | px+3 | 1 Mio | DMEM (10% FBS, 1% P/S, 0,1 mM NEAA, 10µg/mL Blasticidin) | 7 | Elisa Quaas | Rack 04/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| BioCat Company | 06.11.2023 | px+5 | 1 Mio | DMEM (10% FBS, 1% P/S, 0,1 mM NEAA, 10µg/mL Blasticidin) | 10 | Elisa Quaas | Rack 04/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| BioCat Company | 17.11.2023 | px+5 | 1Mio | DMEM (10% FBS, 1% P/S, 0,1 mM NEAA, 10µg/mL Blasticidin) | 7 | Kunpeng Liu | Rack 05/07, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) |
Background
Cultivation and freezing protocols
check data sheet
Data sheets, further information
https://www.cellosaurus.org/CVCL_JY89
http://www.cellbiolabs.com/sites/default/files/AKR-213-gfp-hela-cell-line.pdf
