Virus incl. Viroids & Prions

SARS-CoV2 (attenuated, vaccination strains)

Strain/Type

sCPD9 & sCPD9-ΔFCS

Source/Company

Vetenerian Institute FUB, Jakob Trimpert & Benedikt Kaufer, Kontakt Dusan Kunec (Dusan.Kunec@fu-berlin.de)

Usage Regulation and Limitations

  • for research purposes only
  • only for AG Haag, contact of Katharina Achazi required before use
  • only testing of new therapeutics
  • no vaccine development

How to cite

cite Trimpert J et al. (2021). Cell Rep. 36(5):109493.

Host tropism

human, bat, etc.

Route of Transmission

aerogen (inhalation), air-borne

Characteristics e.g. sensitizing or toxic effects, replication deficiant, not infectious, attenuated, inactivated (upload relevant documents)

causes infection

Approved as biological safety measure if taken as recipient organism for genetic engineering?

no

Genetically modified (GenTSV)

yes, S2

Risk group (BioStoffV)

RG2

Risk assessment

Operation instructions (mandatory for RG2 and higher)

According to ZKBS (888-12-4b LAGeSo-Änderung Genehmigung v. 12.5.22.pdf) and LaGeSo (888-12-4b ZKBS-Stellungnahme v. 5.4.22.pdf)

Occupational health care (according to ArbMedVV)

yes

Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)

sourcevirus strainfreezing datevirus amount per vialstock was produced onno. of aliquotsbelongs to
(full name)

rack/box in N2 tank or -80°C freezer and  location (room, address)


comments

Background

Groenke N et al. (2020). Cell Rep. 31(4):107586.

Trimpert J et al. (2021). Cell Rep. 36(5):109493.

Trimpert J et al. (2021). Sci Adv. 7(49):eabk0172.

Trimpert J et al. (2020). Cell Rep. 8;33(10):108488.

Jackson CB et al. (2021). Biochem Biophys Res Commun. 538:108-115.

Volz E et al. (2021). Cell. 184(1):64-75.e11.

Cultivation and freezing protocols

Transfection assay SOP_JT(1).docx

Data sheets, inactivation cetificate, further information

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