Bacteria
Escherichia coli
Strain/Type
E. coli AR3110, K12 derivative
Genotype: F- λ- rph-1 INV(rrnD, rrnE)
bcsQ allele (codon 6 changed from TAG (stop) to TTG (leucine))
Characteristics e.g. sensitizing or toxic effects, resistance to antibiotics
Approved as biological safety measure if taken as recipient organism for genetic engineering?
yes
Genetically modified (GenTSV)
yes (S1)
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/ecoli/detail.jsf?id=321&dswid=2647&dsrid=921)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menu in the left header to e.g. add a row)
| source | bacterial strain | freezing date | amount of bacteria per vial | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments | ||
|---|---|---|---|---|---|---|---|---|---|
| Humboldt Universität– Das Präsidium (Körperschaft des öffentlichen Rechts) Philippstraße 13, Haus 22, 10115 Berlin | Katharina Achazi (Lingyan Gao) | -80°C "Omega", 115.2 | |||||||
| Robyn Diehn | AR3110 | 31.10.2022 | 500uL | 2 | Tatyana Povolotsky | -80°C "Omega", 115.2 B-2-11 | Frozen stock strain | ||
| Tatyana Povolotsky | AR3110 | 19.10.2023 | 200uL | 10 | Tatyana Povolotsky | -80°C "Omega", 115.2 A-1-1 | 0.3 OD starter cultures frozen in glycerol or DMSO | ||
| Tatyana Povolotsky | AR3110 | April 2024 | 500uL | 1 | Giorgia Mansutti | -80°C "Omega", 115.2 A-1-2 | Frozen stock strain | ||
| Robyn Diehn | AR3110 | 22.07.2022 | 1000µl | 1 | Britt Schaffranietz | -80°C "Omega", 115.2 B-1-25 | Frozen stock strain; 1,0 OD | ||
| Britt Schaffranietz | AR3110 | 21.11.2023 | 500µl | 11 | Britt Schaffranietz | -80°C "Omega", 115.2 B-1-25 | Frozen stock strain |
Background
There are 8 site (9nt) differences between W3110 and MG1655. They reside in 7 orgs and one rRNA gene. Two are nonfunctional (rpoS and dcuA) and 5 are unknown missense mutations. bcsQ allele (codon 6 changed from TAG (stop) to TTG (leucine)).
This strain is used for biofilm formation experiments and is capable of expressing cellulose and curli at temperatures of less than 30°C in LB without salt.
Paper that describes the generation of the strain.
Cultivation and freezing protocols
Strain should ideally be stored in 7% DMSO (930uL overnight culture and 70uL DMSO in cryotube), alternatively can be stored in glycerol stock (25% v/v) (500uL overnight culture and 500uL 50% glycerol), flash-frozen in liquid nitrogen. Long-term storage -80 degrees C and DMSO storage recommended.
