Cell Line
4T1, 4T1-A
Organism
mouse
Tissue/Cell type
mammary gland , epithelial
Genetically modified (GenTSV)
Risk group (BioStoffV), sensitizing or toxic effects
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=911&dswid=2254&dsrid=838)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| ATCC | 04.02.2021 | P3+3 | 6x 10^5 | RPMI + 10% FCS | 10 (storage stock, DO NOT USE, only use working stocks for experiments) | Katharina Achazi, Shaohui Xu | 4/3, N2 tank,(SupraFab, Altensteinstraße 23a, 114.1) | storgae stock: DO NOT USE, use working stock instead tested mycopplasm free (subcultured population) 2021-02-11 |
| ATCC | 13.02.2021 | P3+5 | 6x 10^5 | RPMI + 10% FCS | 20 (working stock) | Katharina Achazi, Shaohui Xu | 10/6, N2 tank,(SupraFab, Altensteinstraße 23a, 114.1) | Working Stock, tested mycopplasm free (subcultured population) 2021-02-11 |
| ATCC |
Cultivation and freezing protocols
Data sheets, further information
https://www.lgcstandards-atcc.org/products/all/CRL-2539.aspx?geo_country=de#generalinformation
wegCell Line
4T1, 4T1-A
Organism
mouse
Tissue/Cell type
mammary gland , epithelial
Genetically modified (GenTSV)
Risk level (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=911&dswid=2254&dsrid=838)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| ATCC | 04.02.2021 | P3+3 | 6x 10^5 | RPMI + 10% FCS | 10 (storage stock, DO NOT USE, only use working stocks for experiments) | Katharina Achazi, Shaohui Xu | 4/3, N2 tank, Arnimallee 22, E004 | storgae stock: DO NOT USE, use working stock instead tested mycopplasm free (subcultured population) 2021-02-11 |
| ATCC | 13.02.2021 | P3+5 | 6x 10^5 | RPMI + 10% FCS | 20 (working stock) | Katharina Achazi, Shaohui Xu | 10/6, N2 tank, Arnimallee 22, E004 | Working Stock, tested mycopplasm free (subcultured population) 2021-02-11 |
Cultivation and freezing protocols
Data sheets, further information
https://www.lgcstandards-atcc.org/products/all/CRL-2539.aspx?geo_country=de#generalinformation
