Cell Line
A431; A431; A431/P
Organism
human
Tissue/Cell type
squamous cell carcinoma, epidermoid carcinoma, epithelial
Genetically modified (GenTSV)
Risk group (BioStoffV), sensitizing or toxic effects
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=652&dswid=1649&dsrid=459)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Mivenion | 20.04.2015 | p34 | DMEM, 10% FBS | 5 | Katharina Achazi | 12/07, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | ||
| Mivenion | 26.10.2016 | p37 | for 25cm2 flask | DMEM (4.5g/l glucose), 10% FBS, 1% P/S | 3 | Katharina Achazi | 04/01, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | tested mycoplasm free before freezing |
| Mivenion | 14.07.2016 | 10 | Yan Li, AG Ma (Katharina Achazi) | 03/10, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | ||||
| Mivenion | 14.01.2020 | p40 | 1000000 | DMEM (4.5g/l glucose), 10% FBS, 1% P/S | 6 | Elisa Quaas | 04/06, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| Mivenion | 25.02.2021 | p53 | 1000000 | DMEM (4.5g/l glucose), 10% FBS, 1% P/S | 5 | Elisa Quaas | 04/06, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) |
Cultivation and freezing protocols
check data sheet
Data sheets, further information
https://www.dsmz.de/collection/catalogue/details/culture/ACC-91
https://www.lgcstandards-atcc.org/products/all/CRL-1555.aspx
Cell Line
A431; A431; A431/P
Organism
human
Tissue/Cell type
squamous cell carcinoma, epidermoid carcinoma, epithelial
Genetically modified (GenTSV)
Risk level (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=652&dswid=1649&dsrid=459)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Mivenion | 20.04.2015 | p34 | DMEM, 10% FBS | 5 | Katharina Achazi | 12/07, N2 tank (Arnimallee 22, E004) | ||
| Mivenion | 26.10.2016 | p37 | for 25cm2 flask | DMEM (4.5g/l glucose), 10% FBS, 1% P/S | 3 | Katharina Achazi | 04/01, N2 tank (Arnimallee 22, E004) | tested mycoplasm free before freezing |
| Mivenion | 14.07.2016 | 10 | Yan Li, AG Ma (Katharina Achazi) | 03/10, N2 tank (Arnimallee 22, E004) | ||||
| Mivenion | 14.01.2020 | p40 | 1000000 | DMEM (4.5g/l glucose), 10% FBS, 1% P/S | 8 | Elisa Quaas | 04/06, N2 tank (Arnimallee 22, E004) | |
| Mivenion | 25.02.2021 | p53 | 1000000 | DMEM (4.5g/l glucose), 10% FBS, 1% P/S | 7 | Elisa Quaas | 04/06, N2 tank (Arnimallee 22, E004) |
Cultivation and freezing protocols
check data sheet
Data sheets, further information
https://www.dsmz.de/collection/catalogue/details/culture/ACC-91
https://www.lgcstandards-atcc.org/products/all/CRL-1555.aspx
