Cell Line

BT-474; BT474; Bt-474; BT474

Organism

human (Homo sapiens)

Tissue/Cell type

breast ductal carcinoma 

Genetically modified (GenTSV)

Risk group (BioStoffV), sensitizing or toxic effects

1

Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)

sourcefreezing datepassage no.cell count per vialcell culture medium incl. supplementsno. of aliquotsbelongs to
(full name)
rack/box in N2 tank or -80°C freezer and  location (room, address)

comments


Charité, Chuanbing Zhang/Jan Eucker24.06.2021px+1500000RPMI+10%FBS4Katharina Achazi (Peng Tang)02/07, N2 tank (SupraFab, Altensteinstraße 23a, 114.1)
DSMZ17.07.2023

80% RPMI 1640 + 20% h.i. FBS + 10 µg/ml human insulin + 2 mM L-glutamine 

1Britt Schaffranietz
01/04/08, N2 tank (SupraFab, Altensteinstraße 23a, 114.1)


Background

HER2 positive according to https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665029/

Cell Micrograph for ATCC HTB-20 BT-474

Cultivation and freezing protocols