Cell Line
CaLu-3; CALU-3; Calu 3; Calu3; CALU3
Organism
human
Tissue/Cell type
lung adenocarcinoma epithelial cell
Genetically modified (GenTSV)
Risk group (BioStoffV), sensitizing or toxic effects
1
Risk assessment
based on ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=415&dswid=6429&dsrid=151)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°C freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| ATTC HTB-55 (Charite, Prof. Dr. med. Andreas C. Hocke (M.Sc.) | 22.07.2021 | px+2 | 1000000 | DMEM with Glutamin and Sodiumpyruvat + 10% FBS + 1% P/S + NEAA | 5 | Katharina Achazi, Elisa Quaas | N2 tank, Rack 04/06 (SupraFab, Altensteinstraße 23a, 114.1) | sensitive and difficult to culture, difficult to detach: subculture at 70%-80% confluent, use always new botttle, wash one time with PBS and 1x with Trypsin before trypsination, senstitive to endotoxins in FBS |
| ATTC HTB-55 (Charite, Prof. Dr. med. Andreas C. Hocke (M.Sc.) | 18.07.2022 | px+13 | 1000000 | DMEM with 10% FBS + 1% P/S | 6 | Elisa Quaas | N2 tank, Rack 04/07 (SupraFab, Altensteinstraße 23a, 114.1) | mycoplasma tested |
| ATTC HTB-55 (Charite, Prof. Dr. med. Andreas C. Hocke (M.Sc.) | 08.03.2024 | px+16 | 1000000 | DMEM with 10% FBS + 1% P/S | 6 | Jingyi Tang | N2 tank, Rack 06/10 (SupraFab, Altensteinstraße 23a, 114.1) | |
| ATTC HTB-55 (Charite, Prof. Dr. med. Andreas C. Hocke (M.Sc.) | 14.03.2024 | px+17 | 1000000 | DMEM with 10% FBS + 1% P/S | 3 | Jingyi Tang | N2 tank, Rack 06/10 (SupraFab, Altensteinstraße 23a, 114.1) |
