Bacteria
Escherichia coli
Strain/Type
E. coli ORN178, K12 derivative
Source/Company
Prof. Ordndorff über Prof. Seeberger, MPIKG (contact Christina Roth: Christian.Roth@mpikg.mpg.de)
Usage Regulation and Limitations
Ask Prof. Seeberger (contact Christina Roth: Christian.Roth@mpikg.mpg.de) for permission to use.
How to cite
Thank Prof. Orndorff and Prof. Seeeberger to provide the bacterial strains.
Characteristics e.g. sensitizing or toxic effect, resistance to antibiotics
E. coli ORN178 binds to mannose-containing receptors on eucaryotic cells and is similar to ORN115 except tetR inserted ca. 200 bp 39 of the end of fimH, Pil1
ORN115: thr-1 leuB thi-1 D(argF-lac)U169 xyl-7 ara-13 mtl-2 gal-6 rpsL tonA2 supE44 pilG1 lr Pil1 (does not exhibit phase variation of piliation)
Approved as biological safety measure if taken as recipient organism for genetic engineering?
yes
Genetically modified (GenTSV)
yes
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/organismen/detail.jsf?id=841&dswid=2450&dsrid=464) and Stellungsnahme der ZKBS Az.: 6790-10-56 (details of the bacteria are discribed in the Harris et al. 2001)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | bacterial strain | freezing date | amount of bacteria per vial | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|
| Jens Dernedde (Charité) | 1 ml | 20 | Katharina Achazi/Elisa Quaas | -80°C "Omega", Shelf B, Rack 1, Box 9, Room 115.2, SupraFAB, Altensteinstraße 23a | |||
| Elisa Quaas | E. coli ORN178 | 1 mL | 1 | Tatyana Povolotsky | -80°C "Omega", 115.2 B-2-11 | Frozen stock strain | |
