Bacteria
Escherichia coli
Strain/Type
E. coli XL1-Blue, K12 derivative
Genotype: endA1 gyrA96(nalR) thi-1 recA1 relA1 lac glnV44 e14- Δ(mcrCB-hsdSMR-mrr)171 recB recJ sbcC umuC::Tn5 uvrC F'[ ::Tn10 proAB+ lacIq Δ(lacZ)M15 Amy CmR]
Characteristics e.g. sensitizing or toxic effect, resistance to antibiotics
recA1, endA1, gyrA96, thi-1, hsdR17, supE44, relA1, lac [F´ proAB lacIqZΔM15 Tn10 (TetR)]
Approved as biological safety measure if taken as recipient organism for genetic engineering?
yes
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/ecoli/detail.jsf?dswid=2647&dsrid=605&id=54)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | bacterial strain | freezing date | amount of bacteria per vial | host cells, stock was produced on | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Agilent | Stefanie Wedepohl | -80°C "Omega", 115.2? | ||||||
Background
nalidixic acid resistant
tetracycline resistant (carried on the F plasmid)
