Bacteria incl. Archaea
Escherichia coli (E.coli)
Strain/Type
C2566 (derivative of BL21)
Source/Company
NEB
Usage Regulation and Limitations
How to cite
Host tropism
Route of Transmission
Characteristics e.g. sensitizing or toxic effects, resistance to antibiotics
T7 RNA Polymerase in the lac operon (no λ prophage), deficient in proteases Lon and OmpT, resistant to phage T1 (fhuA2), does not restrict methylated DNA (McrA-, McrBC-, EcoBr-m-, Mrr-)
Approved as biological safety measure if taken as recipient organism for genetic engineering?
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Operation instructions (mandatory for RG2 and higher)
Occupational health care (according to ArbMedVV)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | bacterial strain | freezing date | amount of bacteria per vial | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°C freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|
| NEB, AG Koksch/Christin Treiber-Kleinke | C2566 | 02.04.2024 | 3 | Christin Treiber-Kleinke | 80°C freezer alpha, C2-2 | suitable for high efficiency transformation and protein expression | |
| Christin Treiber-Kleinke | E. coli C2566 | 27.06.2024 | 1 mL | 5 | Tatyana Povolotsky | -80°C "Omega", 115.2 B-2-11 | suitable for high efficiency transformation and protein expression Frozen stock strain |
Background
Cultivation and freezing protocols
Data sheets, further information
https://www.neb.com/en/products/c2566-t7-express-competent-e-coli-high-efficiency
