Cell Line
HCT-116; HCT 116; HCT.116; HCT_116; HCT116; CoCL2
Organism
human
Tissue/Cell type
colorectal carcinoma, colon carcinoma, epithelial
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=105&dswid=2452&dsrid=597)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Mivenion | 25.11.2019 | p38 | 1000000 | RPMI 1640 10% FBS 1% P/S | 11 | Elisa Quaas | 03/07, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | tested mycoplasm free before freezing |
| Mivenion | 17.12.2015 | p35 | RPMI | 3 | Katharina Achazi (Paul Hillmann) | 03/07, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | ||
Mivenion | 04.09.2017 | p39 | 8,40E+06 | RPMI | 10 | Katharina Achazi (Ali?) | 03/07, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
Cultivation and freezing protocols
check data sheet
Cell Line
HCT-116; HCT 116; HCT.116; HCT_116; HCT116; CoCL2
Organism
human
Tissue/Cell type
colorectal carcinoma, colon carcinoma, epithelial
Genetically modified (GenTSV)
Risk level (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=105&dswid=2452&dsrid=597)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Mivenion | 25.11.2019 | p38 | 1000000 | RPMI 1640 10% FBS 1% P/S | 11 | Elisa Quaas | 03/07, N2 tank (Arnimallee 22, E004) | tested mycoplasm free before freezing |
| Mivenion | 17.12.2015 | p35 | RPMI | 3 | Katharina Achazi (Paul Hillmann) | 03/07, N2 tank (Arnimallee 22, E004) | ||
Mivenion | 04.09.2017 | p39 | 8,40E+06 | RPMI | 10 | Katharina Achazi (Ali?) | 03/07, N2 tank (Arnimallee 22, E004) | |
Cultivation and freezing protocols
check data sheet
