Cell Line
HEK293T; 293T; HEK 293T; HEK-293T; HEK-293-T; HEK 293 T; 293-T; 293 T; Human Embryonic Kidney 293T; 293tsA1609neo
Organism
human
Tissue/Cell type
human embryonic kidney
Genetically modified (GenTSV)
S1
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=2733&dswid=9596&dsrid=863)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| DSMZ | 25.02.2018 | p4 | DMEM, 10%FCS | 20 | Karen Vester, AG Wahl | N2 tank (Takustr. 6, K004) | ||
| 21.06.2019 | 1,00E+06 | DMEM 10% FBS 1% P/S | 8 | Katharina Achazi | ||||
| 20.04.2016 | 9 | Yan Li, AG Ma (Katharina Achazi) | 03/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||||
| Jens Dernedde, Charite (DSMZ) | 30.07.2013 | px+1 | 4 | Katharina Achazi (CK) | 05/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| Uni Leipzig, Michael Schäfer (DSMZ) | 29.10.2014 | p3/p4 | 6 | Katharina Achazi/Andrea Senger, FU Biochemie | 05/03, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | WT | ||
| AG Knaus (DSMZ) | 30.10.2014 | 8 | Katharina Achazi/Andrea Senger, FU Biochemie | 05/03, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | WT | |||
| Jens Dernedde, Charite (DSMZ) | 08.04.2014 | px+3 | 5 | Katharina Achazi (CK) | 12/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| Jens Dernedde, Charite (DSMZ) | 12.11.2014 | px+2 | 14 | Katharina Achazi (CK) | 12/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| Jens Dernedde, Charite (DSMZ) | 26.10.2016 | px+9 | for 25cm2 flask | DMEM (4.5g/l glucose), 10% FBCS, 1% P/S | 7 | Katharina Achazi | 04/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Does not exist anymore? |
| DSMZ | 15.04.2019 | DMEM 10% FBS 1% P/S | 5 | Stefanie Wedepohl | 05/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| DSMZ | 17.11.2021 | DMEM 10% FBS 1% P/S | 4 | Chuanxiong Nie | 04/07, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| DSMZ | DMEM 10% FBS 1% P/S | 2 | Chuanxiong Nie | 6/9, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||||
| DSMZ | 11.14.2023 | 1.8x10^6 cells | DMEM 10% FBS 1% P/S | 8 | Chuanxiong Nie | 6/9, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||
| FU Insitut Fur Virologie | 03.04.2024 | px+1 | 1x10^6 cells | DMEM 10% FBS 1% P/S | 6 | Chuanxiong Nie | 6/8, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) |
Cultivation and freezing protocols
check data sheet
Cell Line
HEK293T; 293T; HEK 293T; HEK-293T; HEK-293-T; HEK 293 T; 293-T; 293 T; Human Embryonic Kidney 293T; 293tsA1609neo
Organism
human
Tissue/Cell type
human embryonic kidney
Genetically modified (GenTSV)
S1
Risk level (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=2733&dswid=9596&dsrid=863)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| DSMZ | 25.02.2018 | p4 | DMEM, 10%FCS | 20 | Karen Vester, AG Wahl | N2 tank (Takustr. 6, K004) | ||
| 21.06.2019 | 1,00E+06 | DMEM 10% FBS 1% P/S | 8 | Katharina Achazi | ||||
| 20.04.2016 | 9 | Yan Li, AG Ma (Katharina Achazi) | 03/08, N2 tank, Arnimallee 22, E004 | |||||
| Jens Dernedde, Charite (DSMZ) | 30.07.2013 | px+1 | 4 | Katharina Achazi (CK) | 05/01, N2 tank, Arnimallee 22, E004 | |||
| Uni Leipzig, Michael Schäfer (DSMZ) | 29.10.2014 | p3/p4 | 6 | Katharina Achazi/Andrea Senger, FU Biochemie | 05/03, N2 tank, Arnimallee 22, E004 | WT | ||
| AG Knaus (DSMZ) | 30.10.2014 | 8 | Katharina Achazi/Andrea Senger, FU Biochemie | 05/03, N2 tank, Arnimallee 22, E004 | WT | |||
| Jens Dernedde, Charite (DSMZ) | 08.04.2014 | px+3 | 5 | Katharina Achazi (CK) | 12/01, N2 tank, Arnimallee 22, E004 | |||
| Jens Dernedde, Charite (DSMZ) | 12.11.2014 | px+2 | 16 | Katharina Achazi (CK) | 12/01, N2 tank, Arnimallee 22, E004 | |||
| Jens Dernedde, Charite (DSMZ) | 26.10.2016 | px+9 | for 25cm2 flask | DMEM (4.5g/l glucose), 10% FBCS, 1% P/S | 7 | Katharina Achazi | 04/01, N2 tank, Arnimallee 22, E004 | Does not exist anymore? |
| DSMZ | 15.04.2019 | DMEM 10% FBS 1% P/S | 5 | Stefanie Wedepohl | 05/05, N2 tank, Arnimallee 22, E004 | |||
Cultivation and freezing protocols
check data sheet
