Cell Line
HER-14 (NIH 3T3 cells expressing human EGFR); NIH3T3-Her14; DHER14; HER 14
Organism
mouse
Tissue/Cell type
fibroblast
Genetically modified (GenTSV)
S1
Risk group (BioStoffV)
1
Risk assessment
risk level 1 as donor (human) and recipient (NIH 3T3 cells, mouse fibroblasts) are risk level 1 and the defined transferred nucleic acids (human EGFR; HGNC:3236) have no hazardous potential
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Charité, Jens Dernedde | 04.04.2016 | px+3 | 8,70E+05 | DMEM HG; 10% FCS; 1% P/S; coat culture flask with 1mg/ml gelatine | 7 | Katharina Achazi (Paul Hillmann) | 03/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| Charité, Jens Dernedde | px+7 | DMEM HG; 10% FCS; 1% P/S; coat culture flask with 1mg/ml gelatine | 9 | Katharina Achazi (Paul Hillmann) | 03/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) |
