Cell Line
HL-60
Organism
homo sapiens, human
Tissue/Cell type
Acute promyelocytic leukemia, promyeloblast from peripheral blood. Lymphoblast morphology
Genetically modified (GenTSV)
no
Risk group (BioStoffV)
1
Risk assessment
according to ZKBS Zelllinienliste
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°C freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| DSMZ (MPI) | 21.3.2022 | P3 | 1E7 | RPMI 10% FBS 1% P/S | 4 | Stefanie Wedepohl | Rack5 Box4 A3-D3 | |
| DSMZ (MPI) | 24.3.2022 | P4 | 1E7 | RPMI 10% FBS 1% P/S | 3 | Stefanie Wedepohl | Rack5 Box4 H4-J4 |
Background
human acute myeloid leukemia established from the peripheral blood of a 35-year-old woman with acute myeloid leukemia (AML FAB M2) in 1976; cells can be used for induction of differentiation studies; described to be responsive to DMSO, phorbol ester TPA and other reagents and to carry amplified c-myc gene; present cells are apparently tetraploid derivates of hypodiploid original where c-myc was amplified in dmin (instead of hsr) confirmed as human with IEF of MDH, NP
Cultivation and freezing protocols
freeze in 10% DMSO in full medium
