Cell Line
HT29-mtx-E12, HT-29-mtx-E12
Organism
human
Tissue/Cell type
epithelial cells from colon adenocarcinoma
Genetically modified (GenTSV)
-
Risk group (BioStoffV)
1
Risk assessment
HT29-mtx-E12 is a subclone of cell line HT29-mtx which is a subclone of cell line HT29
parent cell line HT29 is according to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?dswid=3493&dsrid=2&id=6205) risk level 1
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| ECACC (Sigma-Aldrich) | 03.03.2018 | p2 | 1 Mio | DMEM + FBS+ PS+ NEAA | 1? | Laura Elomaa | 10/1, N2 tank, SupraFAB , 114.1 | |
| ECACC (Sigma-Aldrich) | 23.8.21 | p4 | 1 Mio | DMEM + FBS+ PS+ NEAA | 7 | Johanna Scholz | 10/1, N2 tank, SupraFAB , 114.1 | |
| ECACC (Sigma-Aldrich) | 02.06.22 | p11 | 1 Mio | DMEM + FBS+ PS+ NEAA | 6 | Johanna Scholz | 06/06, N2 tank, SupraFAB 114.1 | |
| ECACC (Sigma-Aldrich) | 9.6.2023 | p13 | 1Mio | DMEM + FBS+ PS+ NEAA | 18 | Johanna scholz | 06/07, N2 tank, SupraFAB 114.1 | |
| ECACC (Sigma-Aldrich) | 28.3.2024 | p20 | 1Mio | DMEM + FBS+ PS+ NEAA | 4 | Johanna Scholz | 06/07, N2 tank, SupraFAB 114.1 | Reihe 2 |
Background
HT29 cells were differentiated into mature goblet cells using methotrexate. Mucous-secreting HT29-MTX subclones were isolated from this cell clone and characterized with regard to tight junction formation, development of confluent monolayers and production of a mucous layer. HT29-MTX-E12 provides a model system to study the influence of the mucous layer on nanoparticle diffusion
