Cell Line
HUVEC, primary HUVEC
Organism
human
Tissue/Cell type
umbilical vein endothelial cells
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=2513&dswid=7455&dsrid=53)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Cellsystem | 11.7.2018 | p3 | 1000000 | VascuLife VEGF Medium Complete Kit | 0 | Johanna Scholz | 13/04, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Catal. no. FC-0003 Lot.no. 04551 |
| Cellsystem | 14.2.2018 | p3 | 1000000 | VascuLife VEGF Medium Complete Kit | 0 | Johanna Scholz | 13/04, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Catal. no. FC-0003
|
| Cellsystem | 26.2.2019 | p3 | 1000000 | VascuLife VEGF Medium Complete Kit | 0 | Johanna Scholz | 13/04, N2 tank,(SupraFab, Altensteinstraße 23a, 114.1) | Catal. no. FC-0003
|
| Cellsystem | 09.06.2020 | p2 | 4x 1000000 1x 760000 | VascuLife VEGF Medium Complete Kit | 0 | Johanna Scholz | 13/04,Reihe 2, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Catal. no. FC-0003, ein vial hat weniger Zellen (ist gekennzeichnet)
|
| Cellsystem | 10.6.2020 | p2 | 1000000 | VascuLife VEGF Medium Complete Kit | 0 | Johanna Scholz | 13/04, Reihe 2, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Catal. no. FC-0003,
|
| Cellsystem | 27.9.2019 | p3 | 1000000 | VascuLife VEGF Medium Complete Kit | 0 | Steffi/ Johanna Scholz | 13/04, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Catal. no. FC-0003
|
| Gibco | 27.01.2016 | P 4 (since me) | 100000 | EBM | 3 | Katharina Achazi (Jianguang) | 03/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Does not exist anymore? |
| Gibco | 09.03.2016 | P9 | 3 | Katharina Achazi (Jianguang) | 03/05, N2 tank,(SupraFab, Altensteinstraße 23a, 114.1) | |||
| Jens Dernedde, Charite (Gibco) | 26.05.2014 | p5 | 4 | Katharina Achazi | 11/02, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| Jens Dernedde, Charite (Gibco) | 06.04.2014 | p6 | 1 | Katharina Achazi | 11/02, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| Jens Dernedde, Charite (Gibco) | 26.06.2014 | p4 | 1 | Katharina Achazi (Paul Hillmann) | 11/02, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| Promocell C-12203/395 Z049 | 07.04.2015 | p8 | 7 | Johanna Scholz (Dennis Müller) | 11/02, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| Cellsystem | 3.11.2021 | p2 | 1000000 | VascuLife VEGF Medium Complete Kit | 1 | Johanna Scholz für AG Haag | 04/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Catal. no. FC-0003
|
| Cellsystem | 30.4.2021 | p4 | 1000000 | VascuLife VEGF Medium Complete Kit | 1 | Johanna Scholz | 13/04 Reihe drei | |
| Cellsystem | 1.12.2021 | p3 | 1000000 | VascuLife VEGF Medium Complete Kit | 3? | Johanna Scholz | 13/04 SupraFab, Altensteinstraße 23a, 114.1) | from confluent flask |
| Cellsystem | 2.9.2022 | p3 | 900000 | VascuLife VEGF Medium Complete Kit | 1 | Johanna Scholz | 13/04 SupraFab, Altensteinstraße 23a, 114.1) | |
| Cellsystem | 09.09.2022 | p4 | 1000000 | VascuLife VEGF Medium Complete Kit | 6 | Johanna Scholz | 06/06, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| Cellsystem | 9.01.2023 | p2 | 1000000 | VascuLife VEGF Medium Complete Kit | 2 | Johanna Scholz | 13/04 SupraFab, Altensteinstraße 23a, 114.1) | Reihe 3,4,5 |
| AG Weinhart | 01.07.2023 | P5 | 300000 | VascuLife VEGF Medium Complete Kit | 7 | Chuanxiong Nie | 06/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | use only until passage 6 |
| Cellsystems | 29.06.2023 | p2 | 1000000 | VascuLife VEGF Medium Complete Kit | 1 | Johanna Scholz | 13/04 | Reihe 2 |
| Cellsystems | 4.9.2023 | p2 | 1000000 | VascuLife VEGF Medium Complete Kit | 1 | Johanna Scholz | 13/04 | |
| Cellsystems | 23.02.2024 | p9 | 1000000 | VascuLife VEGF Medium Complete Kit | 2 | Jingyi Tang | 06/10, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | please use only to passage no 6 |
| Cellsystems | 01.03.2024 | p10 | 1000000 | VascuLife VEGF Medium Complete Kit | 3 | Jingyi Tang | 06/10, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| cellsystems | 22.4.2024 | p3 | 600000 | VascuLife VEGF Medium Complete Kit | 2 | Johanna | 13/04 |
Background
USE ONLY IN AGREEMENT WITH AG WEINHART (Johanna Scholz or Anke Hoppensack)
Cultivation and freezing protocols
Data sheets, further information
https://cellsystems.de/datasheet/c/normal-human-umbilical-vein-endothelial-cells-huvec
Kommentar
Johanna Scholz sagt:
Please use medium only four weeks!!!! Split twice a week.