Cell Line
HaCat; HaCAT; HACAT; Hacat
Organism
human
Tissue/Cell type
keratinozyte
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?dswid=2452&dsrid=594&id=21)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Jens Dernedde, Charite (DSMZ; ACC-771) | 21.04.2016 | 5 | Yan Li, AG Ma (Katharina Achazi) | 03/08, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | ||||
| Jens Dernedde, Charite (DSMZ; ACC-771) | 04.11.2014 | p42 | RPMI +1%NEAA | 4 | Falko Neumann, AG Ma (Katharina Achazi) | 03/04, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | ||
| Jens Dernedde, Charite (DSMZ; ACC-771) | 01.06.2015 | p46 | 4 | Katharina Achazi | 13/05, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |||
| Jens Dernedde, Charite (DSMZ; ACC-771) | 08.10.2019 | p57 | 1000000 | RPMI (10% FBS, 1% PS) | 4 | Elisa Quaas | 04/01, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| Jens Dernedde, Charite (DSMZ; ACC-771) | 07.12.2016 | p54 | RPMI (10% FBS, 1% PS) | 0!!! | Katharina Achazi (Magda Ferraro) | 13/08, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | ||
| Jens Dernedde, Charite (DSMZ; ACC-771) | 16.02.2021 | p59 | 1000000 | RPMI (10% FBS, 1% PS) | 2 | Elisa Quaas | 03/07, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | mycoplasm tested before freezing |
| Jens Dernedde, Charite (DSMZ; ACC-771) | 17.02.2022 | p68 | 1000000 | RPMI (10% FBS, 1% PS) | 4 | Elisa Quaas | 03/07, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| Jens Dernedde, Charite (DSMZ; ACC-771) | 19.12.2022 | p64 | 1000000 | RPMI (10% FBS, 1% PS) | 4 | Elisa Quaas | 03/07, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| Jens Dernedde, Charite (DSMZ; ACC-771) | 24.07.2023 | p101 | 1000000 | DMEM (10% FBS, 1% P/S) | 9 | Elisa Quaas | 08/02, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | mycoplasm tested before freezing |
Cultivation and freezing protocols
check data sheet
Data sheets, further information
https://knowledge.lonza.com/cell?id=56&search=HaCaT
https://www.clsgmbh.de/p800_HaCaT.html
