Cell Line
HeLa; Hela; He La; He-La; Henrietta Lacks cells; Helacyton gartleri
Organism
human
Tissue/Cell type
cervix carcinoma, adeno carcinoma, epithelial
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?dswid=2452&dsrid=667&id=22)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| DSMZ | 05.03.2020 | px+5 | 1000000 | DMEM (4.5g/l glucose), 10% FBCS, 1% P/S | 8 | Elisa Quaas | 05/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | tested mycoplasm free before freezing, strange morphology |
| 09.04.2017 | DMEM | 6 | Jie Chen, AG Ma (Katharina Achazi) | 03/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||||
| DSMZ | 29.02.2016 | P 11 (since me) | 400000 | DMEM 10% FBS 1% P/S | 6 | Yan Li, AG Ma (Katharina Achazi) | 03/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| 04.04.2016 | DMEM 10% FBS 1% P/S | ü | Yan Li, AG Ma (Katharina Achazi) | 03/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||||
| Jens Dernedde, Charite (DSMZ) | 21.07.2014 | p15 | 1 | Katharina Achazi (PW/Jens) | 11/02, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| Mivenion (DSMZ) | 14.05.2013 | DMEM 10% FBS 1% P/S | 2 | Stefanie Wedepohl | 05/04, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| DSMZ | 20.04.2014 | p4 | 1500000 | RPMI, 10%FCS, 1% NEAA | 2 | Katharina Achazi | 11/03, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | 16.10.2015 | p6 | 2000000 | RPMI, 10%FCS, 1% NEAA | 0 | Stefanie Wedepohl | 05/04, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | 28.03.2014 | p6 | RPMI | 1 | Katharina Achazi (CK) | 12/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||
DSMZ | 28.03.2014 | p6 | RPMI | 2 | Katharina Achazi (CK) | 12/03, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||
| DSMZ | 08.09.2020 | p13 | 1000000 | RPMI | 11 | Elisa Quaas | 10/04, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | 15.09.2020 | p16 | 1000000 | DMEM | 2 | Elisa Quaas | 05/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | 03.06.2021 | p20 | 1000000 | DMEM (4.5g/l glucose), 10% FBCS, 1% P/S | 5 | Elisa Quaas | 04/06, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | 29.06.2023 | p14 | 1000000 | RMPI | 4 | Johanna Scholz | 04/10 N2 Tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | 06.11.2023 | p22 | 1000000 | DMEM (4.5g/l glucose), 10% FBCS, 1% P/S | 6 | Elisa Quaas | 04/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | 14.12.2023 | p33 | 1000000 | DMEM (4.5g/l glucose), 10% FBCS, 1% P/S | 5 | Rong Zhou | 06/10, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | tested mycoplasm free before |
Cultivation and freezing protocols
check data sheet
