Cell Line
J774A.1
Organism
mouse
Tissue/Cell type
macrophage
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?dswid=8716&dsrid=645&id=4515)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| DSMZ | 05.05.2016 | DMEM | 10 | Yan Li, AG Ma (Katharina Achazi) | 03/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| DSMZ | 22.05.2016 | DMEM | 5 | Yan Li, AG Ma (Katharina Achazi) | 03/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| DSMZ | 30.06.2011 | p18 | 4 | Falko Neumann, AG Ma (Katharina Achazi) | 11/10, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| DSMZ | 16.01.2019 | p19 | DMEM | 5 | Chuanxiong Nie | 11/1, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||
| DSMZ | 26.10.2016 | px+6 | for 25cm2 flask | DMEM (4.5g/l glucose), 10% FCS, 1% P/S | 2 | Katharina Achazi | 04/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | tested mycoplasm free before freezing |
| DSMZ | 25.10.2021 | px+9 | 1 000 000 | DMEM (4.5g/l glucose), 10% FCS, 1% P/S | 8 | Elisa Quaas | 04/06, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | DMEM (4.5g/l glucose), 10% FCS, 1% P/S | 3 | Chuanxiong Nie | 06/09, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||||
| DSMZ | 10.10.2022 | 1000000 | DMEM (4.5g/l glucose), 10% FCS, 1% P/S | 6 | Peng Tang | 03/07, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) |
