Cell Line

L929

Organism

mouse

Tissue/Cell type

fibroblast

Genetically modified (GenTSV)

Risk group (BioStoffV)

1

Risk assessment

According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=1196&dswid=2452&dsrid=168)

Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)

sourcefreezing datepassage no.cell count per vialcell culture medium incl. supplementsno. of aliquotsbelongs to
(full name)		rack/box in N2 tank or -80°° freezer and  location (room, address)

comments


Charite, Changzhu
08.04.2014px+4
 RPMI 1640 containing 10% FBS and 1% PS at 37 °C and 5% CO25Anke Hoppensack (Shabnam)12/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)
Charite, Changzhu
11.05.2015p8
 RPMI 1640 containing 10% FBS and 1% PS at 37 °C and 5% CO27Anke Hoppensack (Shabnam)12/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)
Charite, Changzhu17.12.2013p17
90% RPMI 1640 + 10% h.i. FBS9Katharina Achazi (CK)05/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)
Charite25.7.22p321Mio95% RPMI+ 5% DMSO2Johanna Scholz

=06/06 N2 Tank SupraFAB

Altensteinstr. 23a


Charite, Changzhu12.12.2022p43120000RPMI 1640 containing 10% FBS and 1% PS4Yanping Long08/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)
Charite, Changzhu19.12.2022p451000000DMEM (10% FBS and 1% PS)10Elisa Quaas04/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)
Charite, Changzhu25.05.2023p751000000DMEM (10% FBS and 1% PS)10Yuhang Jiang04/09, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)mycoplasm tested before freezing
Charite, Changzhu

1000000DMEM (10% FBS and 1% PS)8Shaohui Xu03/07, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1)

Background

Cultivation and freezing protocols

Data sheets, further information

https://www.cellosaurus.org/CVCL_0462

https://www.dsmz.de/collection/catalogue/details/culture/ACC-2