Cell Line
MDA-MB-436; HTB-130; MDA_MB_436; MDA MB 436; MDA-Mb-436; MDA-MB436; MDAMB436; MDA-436; MDA436; MB436; MD Anderson-Metastatic Breast-436
Organism
human
Tissue/Cell type
mammary gland/breast; derived from metastatic site: pleural effusion, adenocarcinoma, pleomorphic with multinucleated component cells
Genetically modified (GenTSV)
no
Risk group (BioStoffV)
1
Risk assessment
ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=6366&dswid=6170&dsrid=244)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Charité, Chuanbing Zhang/Jan Eucker (ATCC HTB-130) | 11.08.2020 | px+7 | 1000000 | DMEM (10% FBS, 1%P/S, 1%Glu) | 6 | Katharina Achazi, Elisa Quaas | 02/07, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | mycoplasm free before freezing |
| Charité, Chuanbing Zhang/Jan Eucker (ATCC HTB-130) | 11.08.2020 | px+7 | 1000000 | Leibovitz 15 (20% FBS, 1%P/S, 1%Glu) | 10 | Katharina Achazi, Elisa Quaas | 03/07, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | wrap Parafilm around the lid, mycoplasm free before freezing |
Background
BRCA1 mutant, sensitive to Topodecan(?), HER2 negative according to https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665029/
