Cell Line
MDCK II; MDCKII; MDCK2; MDCK-2; MDCK Type II; MDCKII-WT
Organism
dog
Tissue/Cell type
kidney, epithelial
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?dswid=1367&dsrid=315&id=5964)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| RKI (ECACC) | 2019.03.20 | p25 | DMEM (4.5g/l glucose), 10% FBCS, 1% P/S | 5 | Chuanxiong Nie | 6/9, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | This is the stock, please don't use it if you can find others. | |
| RKI (ECACC) | 21.07.2022 | p47 | 100000 | DMEM (4.5g/l glucose), 10% FBCS, 1% P/S | 8 | Elisa Quaas | N2 tank (SupraFab, Altensteinstraße 23a, 114.1, Rack 04/07) | mycoplasm tested |
| RKI (ECACC) | 12.12.2022 | p50 | 2200000 | DMEM (4.5g/l glucose), 10% FBCS, 1% P/S | 6 | Yanping Long | 08/01, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| RKI (ECACC) | 2023.05 | p26 | 1:6 split from 10-cm dish | DMEM (4.5g/l glucose), 10% FBCS, 1% P/S | 4 | Chuanxiong Nie | 6/9, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| RKI (ECACC) | 2023.11 | p27 | 1.67 x 10^6 cells | DMEM (4.5g/l glucose), 10% FBCS, 1% P/S | 5 | Chuanxiong Nie/Scarlett Manzke | 6/8, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| RKI (ECACC) | 2024.1 | p32 | 6 x 10^5 cells | DMEM (4.5g/l glucose), 10% FBCS, 1% P/S | 6 | Boyu Zheng | 10/4, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| RKI (ECACC) | 29.01.2024 | P33 | 1 x 10^6 cells | DMEM (4.5g/l glucose), 10% FBCS, 1% P/S | 6 | Jingyi Tang | 6/10, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) |
