Cell Line
NIH3T3, NIH-313
Organism
mouse
Tissue/Cell type
embryonic fibroblasts
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?dswid=6725&dsrid=343&id=33)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| ATCC | 25.8.2015 | px+ 2 | 1000000 | DMEM (4,5 g/l Glc) + 10 % FBS (PAN Biotech #P30-3306, Lot P150722 | 2 | Anke Hoppensack | 13/7, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Master cell bank |
| ATCC | 31.8.2015 | px +4 | 500000 | DMEM (4,5 g/l Glc) + 10 % FBS (PAN Biotech #P30-3306, Lot P150722 | 3 | Anke Hoppensack (Melanie Uckert) | 13/7, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Master cell bank |
| ATCC | 03.05.2016 | px +6 | 1000000 | DMEM (4,5 g/l Glc) + 10 % FBS (PAN Biotech #P30-3306, Lot P150722 | 8 | Anke Hoppensack (Melanie Uckert) | 13/7, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Master cell bank |
| ATCC | 10.05.2016 | px +8 | 1000000 | DMEM (4,5 g/l Glc) + 10 % FBS (PAN Biotech #P30-3306, Lot P150722 | 11 | Anke Hoppensack (Melanie Uckert) | 13/3, N2 tank,(SupraFab, Altensteinstraße 23a, 114.1) | wo P/S |
| ? | 09.03.2020 | p21 | 1000000 | DMEM 10% FBS 1% P/S | 5 | Elisa Quass | 05/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| Jens Dernedde, Charite (DSMZ) | 07.11.2016 | P 3 (since me) | 100000 | DMEM 10% FBS 1% P/S | 3 | Katharina Achazi (Jianguang) | 03/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| ATCC | 22.04.2015 | px+5 | 1000000 | 16 | Anke Hoppensack | 03/02, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||
| Jens Dernedde, Charite (DSMZ) | 03.12.2012 | px+47 | 2 | Katharina Achazi(CK) | 05/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| Mivenion (DSMZ) | 16.12.2013 | px | DMEM 10% FBS 1% P/S | 1 | Stefanie Wedepohl | 05/04, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||
| Mivenion (DSMZ) | 06.08.2014 | px+2 | DMEM 10% FBS 1% P/S | 7 | Stefanie Wedepohl | 05/04, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||
| ATCC | 16.01.2015 | p4 | 6 | Anke Hoppensack (Silke Heinen) | 11/06, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| Charité - University Medicine | 04.04.2016 | px+3 | 9,00E+05 | DMEM HG; 10% FCS; 1% P/S | 2 | Katharina Achazi (Paul Hillmann) | 03/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| Charité - University Medicine | 06.06.2016 | px+7 | DMEM HG; 10% FCS; 1% P/S | 5 | Katharina Achazi (Paul Hillmann) | 03/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | positive mycoplasm test, maybe all contaminated? | |
| ? | 25.03.2017 | p8 | 1,00E+06 | DMEM | 7 | Mingjun Li, AG Ma (Katharina Achazi) | 03/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| ? | 11.01.2017 | 4,00E+05 | 6 | Yan Li, AG Ma (Katharina Achazi) | 03/10, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| Jens Dernedde, Charite (DSMZ) | DMEM HG; 10% FCS; 1% P/S | 6 | Chuanxiong Nie | 6/9, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) |
Background
Cultivation and freezing protocols
Data sheets, further information
