Cell Line
RAW 264.7
Organism
mouse
Tissue/Cell type
monocyte/macrophage
Genetically modified (GenTSV)
no
Risk group (BioStoffV), sensitizing or toxic effects
2
Risk assessment
According to ZKBS: https://zag.bvl.bund.de/zelllinien/detail.jsf?dswid=8715&dsrid=649&id=123
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°C freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Marten Kagelmacher (Charité, AG Dernedde) | 11.09.2023 | p8 | 2000000 Z/mL | DMEM (10% FCS, 1% P/S) | 1 | Elisa Quaas | 04/08, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| Marten Kagelmacher (Charité, AG Dernedde) | 11.01.2024 | p9 | 2000000Z/ml | DMEM (10% FCS, 1% P/S) | 5 | Johanna Scholz | 04/08, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | Freezed in 90% FBS 10% DMSO First try of freezing :) Split cells after 3 days with confluency of 70-80 %. |
| Marten Kagelmacher (Charité, AG Dernedde) | 18.1.2024 | ´p11 | 2000000 | DMEM (10% FCS, 1% P/S) | 1 | Johanna Scholz | 04/08, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| Marten Kagelmacher (Charité, AG Dernedde) | 30.01.2024 | P19 | 1000000 | DMEM (10% FCS, 1% P/S) | 10 | Jingyi Tang | 06/10, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| Marten Kagelmacher (Charité, AG Dernedde) | 27.06.2024 | p18 | 1000000 | DMEM (10% FCS, 1% P/S | 10 | Elisa Quaas | 05/08, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) |
Background
Cultivation and freezing protocols
Data sheets, further information
https://cls.shop/de/RAW-264.7/400319
https://www.cellosaurus.org/CVCL_0493
