Cell Line
RT-4, RT4, RT4P
Organism
human
Tissue/Cell type
urinary bladder transitional cell carcinoma, epithelial
Genetically modified (GenTSV)
no
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?dswid=9879&dsrid=913&id=2535)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°C freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
DSMZ no.: | 30.08.2021 | px+3 | 1000000 | RPMI + 10%FCS + 1% P/S | 8 | Elisa Quaas/Katharina Achazi | 04/06, N2 Tank, (SupraFab, Altensteinstraße 23a, 114.1) | confluency after 2 weeks Growing slowly!!!! |
DSMZ no.: ACC 412 | 12.06.23 | px+4 | 2000000 | RPMI + 10%FCS + 1% P/S | 4 | Johanna Scholz | 04/08 N2 Tank, (SupraFab, Altensteinstraße 23a, 114.1) | after 1 Week they are confluent |
Cultivation and freezing protocols
Data sheets, further information
https://www.dsmz.de/collection/catalogue/details/culture/ACC-412
