Cell Line
SH-SY5Y, SY5Ywt
Organism
human
Tissue/Cell type
bone marrow, epithelial, neuroblastoma, neuroblast-like
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?dswid=4317&dsrid=314&id=91)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Pharmazie, Christina Fischer, AG Rademann (Sigma) | 31.10.2016 | P18 | for 25cm2 flask | DMEM (4.5g/l glucose), 20% FBCS, 1% P/S | 11 | Katharina Achazi | 04/01, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | tested mycoplasm free before freezing |
| Andrea Senge, FU Biochemie (DSMZ) | 22.03.2012 | DMEM/HAM´s F12 mit 10% FCS; 1% PS; 6ml Glutamin; 6ml Non essential Aminoacids (NEAA) | 1 | Katharina Achazi (CK) | 05/01, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |||
| Andrea Senge, FU Biochemie (DSMZ) | 15.10.2012 | p6 | DMEM/HAM´s F12 mit 10% FCS; 1% PS; 6ml Glutamin; 6ml Non essential Aminoacids (NEAA) | 9 | Katharina Achazi (CK) | 05/01, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | ||
| Andrea Senge, FU Biochemie (DSMZ) | 10.11.2014 | DMEM/HAM´s F12 mit 10% FCS; 1% PS; 6ml Glutamin; 6ml Non essential Aminoacids (NEAA) | Andrea Senger, FU Biochemie (Katharina Achazi) | 05/03, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | ||||
| Andrea Senge, FU Biochemie (DSMZ) | 10.11.2014 | 8 | Andrea Senger, FU Biochemie (Katharina Achazi) | 05/03, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | ||||
