Cell Line
U937, U-937
Organism
human
Tissue/Cell type
histiocytic lymphoma (suspension cell line)
Genetically modified (GenTSV)
no
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=2497&dswid=4317&dsrid=704)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Stefanie Wedepohl (Charité) | 15.04.2013 | p8 | RPMI 10% FBS, 1% P/S | 1 | Stefanie Wedepohl | 05/04, N2 tank,(SupraFab, Altensteinstraße 23a, 114.1) | round to polygonal, single cells in suspension | |
| Stefanie Wedepohl | 23.8.2023 | p11 | 1E6 | RPMI 10% FBS, 1% P/S | 18 | Stefanie Wedepohl | 05/04, N2 tank,(SupraFab, Altensteinstraße 23a, 114.1) | round to polygonal, single cells in suspension derived from the stock above |
Chaitanya | 27.05.2017 | px | DMEM, 10% FBS, 1%P/S | 2 | Chuanxiong Nie | 06/09, N2 tank,(SupraFab, Altensteinstraße 23a, 114.1) |
round but strongly adherent | |
| Chaitanya | 24.8.2023 | px+1 | 3E6 | DMEM, 10% FBS, 1%P/S | 5 | 06/09, N2 tank,(SupraFab, Altensteinstraße 23a, 114.1) | strongly adherent, cells were scraped because trypsinizing was not succesful. Derived from the stocks above | |
| Chaitanya | 24.8.2023 | px+1 | 3E6 | RPMI 10% FBS, 1% P/S | 5 | 06/09, N2 tank,(SupraFab, Altensteinstraße 23a, 114.1) | strongly adherent, cells were scraped because trypsinizing was not succesful. Derived from the stocks above | |
| Stefanie Wedepohl (Charité) | 12.09.2023 | ? | 1 Mio/mL | RPMI 10% FBS, 1% P/S | 7 | Elisa Quaas | 04/08, N2 tank,(SupraFab, Altensteinstraße 23a, 114.1) |
Background
Cultivation and freezing protocols
suspension cell line: round to polygonal, single cells in suspension
