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Altensteinstr. 23, 114.4
Responsible Person:
- Stefanie Wedepohl, mail: stefanie.wedepohl@fu-berlin.de
- Markus Bardua, mail: markus.bardua@fu-berlin.de
Type of measurement:
Flow cytometry (FCM) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer.
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FL1: 533/30 nm (e.g. FITC/GFP)
FL2: 585/40 nm (e.g. PE/PI)
FL3: > 670 nm (e.g. PerCP, PerCP-Cy™5.5, PE-Cy7)
FL4: 675/25nm (e.g. APC)
Requirements:
- Before use, Marie Weinhart needs to be contacted to get the permission to use the device
- All users must gain permission to work in the Biolabs and access to the “Zellbio-users” network drive by attending the Biosafety instructions (--> Katharina Achazi)
- All users must get an introduction to the device by the responsible person (see OpenIRIS)
- Booking via OpenIRIS (protocls see here)
Instruction/Manual:
Serial No.:
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