Seitenhistorie

Location:

Altensteinstr. 23a, 114.4

Responsible Person:

• see OpenIRIS (use and registration see here)

Type of measurement:

Flow cytometry (FCM) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer.

Uses for flow cytometry include:

• Cell counting
• Determining cell characteristics and function
• Detecting microorganisms
• Biomarker detection
• Protein engineering detection
• Diagnosis of health disorders such as blood cancers
• Note: Cell sorting is not possible with this device

A flow cytometry analyzer is an instrument that provides quantifiable data from a sample. Other instruments using flow cytometry include cell sorters which physically separate and thereby purify cells of interest based on their optical properties.

This flow cytometer is equipped with 3 lasers and several detectors.

Requirements:

• All users must gain permission to work in the Biolabs and access to the “Zellbio-users” network drive by attending the Biosafety instructions (--> Katharina Achazi)
• All users must get an introduction to the device by the responsible person (see OpenIRIS)
  
• Booking via OpenIRIS (protocls see here)

Instruction/Manual:

Software: Analysis PC & Software

1-Training manual and User Guides

2-Application Notes

3-Additional Documents

4-Additional Protocols

Serial No.:

FUB Inventory No.:

Safety Operation Instruction (German):