Bacteria
Escherichia coli
Strain/Type
E. coli XL1-Blue, K12 derivative
Characteristics e.g. sensitizing or toxic effect, resistance to antibiotics
recA1, endA1, gyrA96, thi-1, hsdR17, supE44, relA1, lac [F´ proAB lacIqZΔM15 Tn10 (TetR)]
Approved as biological safety measure if taken as recipient organism for genetic engineering?
yes
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/ecoli/detail.jsf?dswid=2647&dsrid=605&id=54)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | bacterial strain | freezing date | amount of bacteria per vial | host cells, stock was produced on | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Agilent | Stefanie Wedepohl | -80°C "Omega", 115.2? | ||||||
Background
Used for production of recombinant proteins under the control of T7 RNA polymerase.
The strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq. IPTG is required to induce expression of the T7 RNA polymerase. Protein expression from transforming plasmids containing a T7 promoter-driven expression system is repressed until IPTG induction of T7 RNA polymerase expression occurs. The strain does not contain the lon protease and is also deficient in the outer membrane protease, OmpT. The lack of two key proteases reduces the degradation of heterologous proteinsexpressed in the strain.
