Bacteria
Escherichia coli
Strain/Type
E. coli XL1-Blue, K12 derivative
Genotype: recA1 endA1 gyrA96 (nalR) thi-1 recA1 relA1 lac glnV44 e14- Δ(mcrCB-hsdSMR-mrr)171 recB recJ sbcC umuC::Tn5 uvrC F'[ ::Tn10 proAB+ lacIq Δ(lacZ)M15 Amy CmR] hsdR17 supE44 relA1 lac [F´ proAB lacIqZΔM15 Tn10 (Tetr)]
Characteristics e.g. sensitizing or toxic effect, resistance to antibiotics
XL-1 cells are tetracycline resistant. XL1-Blue cells are endonuclease (endA) deficient, which greatly improves the quality of miniprep
DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the
EcoK endonuclease system. The lacIqZΔM15 gene on the F´ episome allows blue-white color screening.recA1, endA1, gyrA96, thi-1, hsdR17, supE44, relA1, lac [F´ proAB lacIqZΔM15 Tn10 (TetR)]
Approved as biological safety measure if taken as recipient organism for genetic engineering?
yes
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/ecoli/detail.jsf?dswid=2647&dsrid=605&id=54)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | bacterial strain | freezing date | amount of bacteria per vial | host cells, stock was produced on | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Agilent | XL-1B | 21.1.2016 | nd | 21.1.2016 | 1 | Stefanie Wedepohl | -80°C "Omega", 115.2 Shelf B Rack 6 Box 1 H1 | glycerol stock |
| Agilent | XL-1B | var. | nd | 21.1.2016 | var. | Stefanie Wedepohl | -80°C "Omega", 115.2 Shelf B Rack 6 Box 1 row 3 | varying number of aliquots of chemically competent cells prepared from glycerol stock |
| Stefanie Wedepohl | XL-1B | 100 µL | 25 | Robyn Diehn | -80°C "Omega", 115.2, B-2-2 | chemical competent | ||
| Stefanie Wedepohl | XL-1B | 4 | Robyn Diehn | -80°C "Omega", 115.2, B-2-5 | stock | |||
| Stefanie Wedepohl | XL-1B | 1 mL | 4 | Robyn Diehn | -80°C "Omega", 115.2, B-2-17 | With Plasmid: SekTagV4_NT5B | ||
| Stefanie Wedepohl | XL-1B | 1 mL | 4 | Robyn Diehn | -80°C "Omega", 115.2 ?, B-2-17 | With Plasmid: SekTagV4_O-glyko_620_direkt | ||
| Stefanie Wedepohl | XL-1B | 1 mL | 4 | Robyn Diehn | -80°C "Omega", 115.2, B-2-17 | With Plasmid: SekTagV4_O-glyko_1800_direkt | ||
| Stefanie Wedepohl | XL-1B | 1 mL | 05.12.23 | 3 | Robyn Diehn | -80°C "Omega", 115.2, B-2-17 | With Plasmid: SekTagV5.1_D3_CO | |
| Stefanie Wedepohl | XL-1B | 1 mL | 05.12.23 | 3 | Robyn Diehn | -80°C "Omega", 115.2, B-2-17 | With Plasmid: SekTagV5.4_NT5B_delta cys 1088/1130 ala Kol 5 | |
| Stefanie Wedepohl | XL-1B | 1 mL | 12.12.23 | 3 | Robyn Diehn | -80°C "Omega", 115.2, B-2-17 | With Plasmid: SekTagV5.4_D3_delta cys 1088/1130 ala | |
| Stefanie Wedepohl | XL-1B | 1 mL | 12.12.23 | 3 | Robyn Diehn | -80°C "Omega", 115.2, B-2-17 | With Plasmid: SekTagV5.4_o-glyko 620_6x His | |
| Stefanie Wedepohl | XL-1B | 1 mL | 26.01.24 | 4 | Robyn Diehn | -80°C "Omega", 115.2, B-2-17 | With Plasmid: SekTagV4_O-glyko_620_direkt | |
| Stefanie Wedepohl | XL-1B | 1 mL | 26.01.24 | 4 | Robyn Diehn | -80°C "Omega", 115.2, B-2-17 | With Plasmid: SekTagV4_O-glyko_1800_direkt | |
| Stefanie Wedepohl | XL-1B | 1 mL | 26.01.24 | 4 | Robyn Diehn | -80°C "Omega", 115.2, B-2-17 | With Plasmid: SekTagV4_Mini 2 | |
| Stefanie Wedepohl | XL-1B | 200 µL | 21.03.24 | 8 | Robyn Diehn | -80°C "Omega", 115.2, B-2-17 | With Plasmid: SekTagV4_D3_CO | |
| Stefanie Wedepohl | XL-1B | 200 µL | 21.03.24 | 8 | Robyn Diehn | -80°C "Omega", 115.2, B-2-17 | With Plasmid: SekTagV4_D3_CO delta cys 1088/1130 ala | |
| Stefanie Wedepohl | XL-1B | 1 mL | 4 | Robyn Diehn | -80°C "Omega", 115.2, B-2-8 | Stock | ||
| Stefanie Wedepohl | XL-1B | 1 mL | 05.03.24 | 3 | Robyn Diehn | -80°C "Omega", 115.2, B-2-8 | Stock with Tetracycline grown | |
Background
nalidixic acid resistant
tetracycline resistant (carried on the F plasmid)
Cultivation and freezing protocols
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Data sheets, further information
View file name XL-1B.pdf height 250
