Cell Line
L929
Organism
mouse
Tissue/Cell type
fibroblast
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=1196&dswid=2452&dsrid=168)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Charite, Changzhu | 08.04.2014 | px+4 | RPMI 1640 containing 10% FBS and 1% PS at 37 °C and 5% CO2 | 5 | Anke Hoppensack (Shabnam) | 12/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||
| Charite, Changzhu | 11.05.2015 | p8 | RPMI 1640 containing 10% FBS and 1% PS at 37 °C and 5% CO2 | 9 | Anke Hoppensack (Shabnam) | 12/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||
| Charite, Changzhu | 17.12.2013 | p17 | 90% RPMI 1640 + 10% h.i. FBS | 9 | Katharina Achazi (CK) | 05/01, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||
| Charite | 25.7.22 | p32 | 1Mio | 95% RPMI+ 5% DMSO | 5 | Johanna Scholz | =06/06 N2 Tank SupraFAB Altensteinstr. 23a |
Background
Cultivation and freezing protocols
Data sheets, further information
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