In this example, unstained cells are detected based on their morphology by multi-template matching. A representative cell is selected (the template) and all structures on the image that have a certain similarity to the template are identified. This is a simple method that does not require deep programming knowledge. However, it is limited to recognizing only objects that are morphologically very similar.
Click on the image to start the tutorial video 
The original publication can be found here: https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-020-3363-7
